CAJ | 학술논문

为建立猫须草药材HPLC指纹图谱分析方法，采用高效液相色谱法，以Phenomenex Synergi 4u hydro-RP 250×4.60 mm为色谱柱，以甲醇-0.1%甲酸溶液为流动相梯度洗脱，检测波长254 nm，流速1.0 mL·min-1，柱温40℃。结果表明，建立的猫须草药材HPLC指纹图谱，确定了15个共有峰，各猫须草样品指纹图谱与对照指纹图谱的相似度均在0.9以上。该方法简单、准确、重复性好，为更好地控制猫须草药材质量提供有效可靠的方法。
위건립묘수초약재HPLC지문도보분석방법，채용고효액상색보법，이Phenomenex Synergi 4u hydro-RP 250×4.60 mm위색보주，이갑순-0.1%갑산용액위류동상제도세탈，검측파장254 nm，류속1.0 mL·min-1，주온40℃。결과표명，건립적묘수초약재HPLC지문도보，학정료15개공유봉，각묘수초양품지문도보여대조지문도보적상사도균재0.9이상。해방법간단、준학、중복성호，위경호지공제묘수초약재질량제공유효가고적방법。
To establish a method of fingerprint analysis onClerodendranthus spicatusby HPLC, the chromatographic fingerprint was obtained by Phenomenex Synergi 4u hydro-RP 250×4.60 mm with the gradient elution solvent system composed of methanol-0.1% formic acid. The detective wavelength was 254 nm; the flow rate was 1.0 mL·min-1 and the column temperature was maintained at 40℃. The results showed that 15 co-possessing peaks were selected as the fingerprint peaks ofC. spicatus and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples ofC. spicatusand the standard fingerprint. The method with good reproducibility was simple and accurate, it could be used as a effective quality control method forC. spicatus.