CAJ | 학술논문

目的：了解广东清远地区妇女人乳头瘤病毒（HPV）感染基因型的分布情况，为本地区宫颈癌的防治提供科学依据。方法采用核酸分子快速导流杂交基因芯片分型技术对23983例就诊者进行下生殖道HPV 21种基因亚型的分型检测。对其中部分HPV感染者进行宫颈液基细胞检查和（或）在阴道镜下取组织活检进行病理检查。结果23983例标本中，HPV阳性为5372例，阳性率为22．40％，21种基因亚型均有检出，感染率前五位的HPV基因亚型由高到低依次为HPV-16（6.02％）、52（4.46％）、56（2.68％）、11（2.25％）、66（1.86％）。在5372例阳性标本中，单一基因亚型感染的3966例，占73.83％；多重感染的为1406例，比例为26.17％。 HPV感染者高峰年龄段为26～45岁。随宫颈疾病及癌前病变程度的加重，高危型 HPV 感染率均显著上升， CIN Ⅱ、CIN Ⅲ和侵润癌均为100％。结论本地区宫颈炎患者的HPV感染率较高，以16、52、56亚型为主。 HPV感染高发于中青年妇女，宫颈病变程度与HPV感染密切相关，长期持续高危型HPV感染可能导致宫颈癌。进行HPV感染者长期的跟踪随访和预后判断，对降低宫颈癌的发病率等有着重要的意义。
목적：료해엄동청원지구부녀인유두류병독（HPV）감염기인형적분포정황，위본지구궁경암적방치제공과학의거。방법채용핵산분자쾌속도류잡교기인심편분형기술대23983례취진자진행하생식도HPV 21충기인아형적분형검측。대기중부분HPV감염자진행궁경액기세포검사화（혹）재음도경하취조직활검진행병리검사。결과23983례표본중，HPV양성위5372례，양성솔위22．40％，21충기인아형균유검출，감염솔전오위적HPV기인아형유고도저의차위HPV-16（6.02％）、52（4.46％）、56（2.68％）、11（2.25％）、66（1.86％）。재5372례양성표본중，단일기인아형감염적3966례，점73.83％；다중감염적위1406례，비례위26.17％。 HPV감염자고봉년령단위26～45세。수궁경질병급암전병변정도적가중，고위형 HPV 감염솔균현저상승， CIN Ⅱ、CIN Ⅲ화침윤암균위100％。결론본지구궁경염환자적HPV감염솔교고，이16、52、56아형위주。 HPV감염고발우중청년부녀，궁경병변정도여HPV감염밀절상관，장기지속고위형HPV감염가능도치궁경암。진행HPV감염자장기적근종수방화예후판단，대강저궁경암적발병솔등유착중요적의의。
Objective To investigate the distribution of different Human papillomavirus ( HPV) subtype infection of women in parts of Qingyuan City Guangdong Province .In order to offer some evidences for the prevention and treatment of the cervical cancer in the region.Methods Flow-through hybridization and gene chip ( HybriMax) were utilized to detect 21 different subtypes of HPV from low genitalia in 23 983 patients.And some of positive samples were checked for liquid -based pap test(LPT) and(or) biopsy by the vaginoscope.Results 5 372 positive samples were detected, occupying 22.40%in to-tal (5 372/23 983) and covering all 21 genotypes.The order of prevalence rates from high to low were HPV-16(6.02%)、52(4.46%)、56(2.68%)、11(2.25%)、66(1.86%).In 5 372 positive samples, mono-infections account for 3 966, reaching 73.83%;the rest are multiple infections.The peak age of HPV infection ranged from 26 to 45 years old.The rate of high-risk HPV infections were increased with the higher severity of cervical dysplasia , there were all 100%for CINⅡ、CINⅢand invasive cervix carcinoma. Conclusion HPV prevalence is at a high level in woman in Qingyuan region .All 21 genotypes were found in HPV infective women, and majority infection was HPV -16、52、56.HPV infection often occurs in young and middle -age women.There is a close relation between HPV infection and cervix diseases.Cervical carcinoma would caused by HPV infec-ting.Long-time following-up visit for HPV infected patients will reduces the incidence of cervical carcinoma.