现代医院
現代醫院
현대의원
MODERN HOSPITAL
2014年
8期
82-84
,共3页
四妙勇安汤%提取工艺%正交试验%绿原酸%哈巴俄苷
四妙勇安湯%提取工藝%正交試驗%綠原痠%哈巴俄苷
사묘용안탕%제취공예%정교시험%록원산%합파아감
Simiaoyongan decoction%Extracting technology%Orthogonal Test%Chlorogenic acid%Harpagoside
目的：优选四妙勇安汤组方提取工艺。方法以绿原酸和哈巴俄苷两种成分含量之和为考察指标，采用L9（34）正交试验设计筛选最佳提取工艺，测定方法采用HPLC法。结果以两种成分含量之和计，正交试验各因素对实验结果影响大小为提取溶剂＞提取次数＞溶剂量＞提取时间。结论四妙勇安汤最佳提取工艺为用10倍量50％乙醇回流1 h，提取3次。
目的：優選四妙勇安湯組方提取工藝。方法以綠原痠和哈巴俄苷兩種成分含量之和為攷察指標，採用L9（34）正交試驗設計篩選最佳提取工藝，測定方法採用HPLC法。結果以兩種成分含量之和計，正交試驗各因素對實驗結果影響大小為提取溶劑＞提取次數＞溶劑量＞提取時間。結論四妙勇安湯最佳提取工藝為用10倍量50％乙醇迴流1 h，提取3次。
목적：우선사묘용안탕조방제취공예。방법이록원산화합파아감량충성분함량지화위고찰지표，채용L9（34）정교시험설계사선최가제취공예，측정방법채용HPLC법。결과이량충성분함량지화계，정교시험각인소대실험결과영향대소위제취용제＞제취차수＞용제량＞제취시간。결론사묘용안탕최가제취공예위용10배량50％을순회류1 h，제취3차。
Objective To research the best extracting technology of simiaoyongan decoction.Methods As the total contents of chlorogenic acid and harpagoside to be evaluation indicator, adopted L9 (34 ) orthogonal design to select out the best extraction.The main determination was by HPLC method.Results Accounted by the total contents of the two composi-tions, the influential effects of the experimental results were extraction solvent >reflux times >the volume of extraction>the circumfluence time.Conclusion The best extracting technology is adding 10-fold times of 50% alcohol, percolating 1 hours, and three times.
