CAJ | 학술논문

以多倍体啤酒酵母菌株S6作为出发菌株，利用同源重组的方法构建了尿嘧啶营养缺陷型啤酒酵母菌株S6-△U，使之用于遗传标记，并把缺陷型菌株恢复成原营养型S6'。同时对出发菌株S6，缺陷型菌株S6-△U和恢复后的菌株S6'的生长状况进行比较。结果表明，URA 3点突变并没有弱化菌株的生长状况。从而建立一种快速获得缺陷型啤酒酵母菌株的方法。
이다배체비주효모균주S6작위출발균주，이용동원중조적방법구건료뇨밀정영양결함형비주효모균주S6-△U，사지용우유전표기，병파결함형균주회복성원영양형S6'。동시대출발균주S6，결함형균주S6-△U화회복후적균주S6'적생장상황진행비교。결과표명，URA 3점돌변병몰유약화균주적생장상황。종이건립일충쾌속획득결함형비주효모균주적방법。
Polyploid brewer's yeast strain S6 was used as the starting strain to construct uracil auxotrophic strain S6-△U through gene homologous recombination, which could be used as a genetic marker. Then uracil auxotrophic strain S6-△U was restored to original strain and named strain S6'. The growth of strain S6, strain S6-△U and strain S6' was compared and the results suggested that the mutation of URA3 gene would not re-duce the growth of the strain. Accordingly, a method for rapid preparation of auxotrophic brewer's strains had been established in this study.