CAJ | 학술논문

研究温度对酒精酵母GJ2008果糖与葡萄糖混合发酵过程的影响，旨在为甘蔗汁酒精发酵提供参考和依据。在20～36℃温度条件下，以YPDF基础培养基模拟甘蔗汁进行酒精发酵，测定发酵过程中各参数的变化，并对果糖与葡萄糖消耗过程进行曲线拟合，从拟合方程计算出果糖与葡萄糖代谢一半和代谢完全所需的时间。结果表明，温度为20～36℃时，酵母GJ2008会始终优先利用葡萄糖，果糖的利用一直受到葡萄糖的竞争性抑制，发酵液中葡萄糖含量较低时，果糖受葡萄糖的竞争性抑制得到了解除，果糖的利用速率急剧加快。总糖含量为230 g/L左右，GJ2008果糖与葡萄糖混合发酵后期果糖代谢缓慢的主要原因是由于葡萄糖的竞争性抑制作用，而非乙醇的抑制作用。温度对果糖代谢的影响小于对葡萄糖代谢的影响，较低温度对果糖后期代谢缓慢现象有所缓解。最适发酵温度为28℃，发酵时间为28 h，乙醇产率为3.89 g/L·h。
연구온도대주정효모GJ2008과당여포도당혼합발효과정적영향，지재위감자즙주정발효제공삼고화의거。재20～36℃온도조건하，이YPDF기출배양기모의감자즙진행주정발효，측정발효과정중각삼수적변화，병대과당여포도당소모과정진행곡선의합，종의합방정계산출과당여포도당대사일반화대사완전소수적시간。결과표명，온도위20～36℃시，효모GJ2008회시종우선이용포도당，과당적이용일직수도포도당적경쟁성억제，발효액중포도당함량교저시，과당수포도당적경쟁성억제득도료해제，과당적이용속솔급극가쾌。총당함량위230 g/L좌우，GJ2008과당여포도당혼합발효후기과당대사완만적주요원인시유우포도당적경쟁성억제작용，이비을순적억제작용。온도대과당대사적영향소우대포도당대사적영향，교저온도대과당후기대사완만현상유소완해。최괄발효온도위28℃，발효시간위28 h，을순산솔위3.89 g/L·h。
To solve the problems associated with sluggish and stuck sugarcane fermentation due to high levels of residual fructose, it is necessary to find the reasons why yeast stop using fructose during late fermentation stage. In the experiments, YPDF culture medium was used to simulate ethanol fermentation by sugarcane juice at six different temperatures ranging from 20 ℃ to 36 ℃, and the change of each parameter along the fermentation process was determined, then curve fitting of fructose consumption and glucose consumption was done, and complete metabolism/half metabolism time of glucose/fructose was calculated through the fitting equation. We found that temperature had an important impact on fructose and glucose consumption, S.cerevisiae GJ2008 always showed a slightly higher preference for glucose than fructose at temperature ranging from 20 ℃ to 36 ℃, but once glucose was low enough, fructose consumption sped up drastically due to the competitive inhibition released by glucose. The main reason why fructose utilization stuck we could explain is the competitive inhibition produced by the presence of glucose during the late stage of fructose and glucose co-fermentation. Meanwhile, fructose was less strongly affected by temperature decrease and lower temperature could relieve sluggish phenomenon. The optimum temperature for maximum ethanol production in YPDF medium was at 28℃ (fermenting time was 28 h) with ethanol yield of 3.89 g/L?h. Those data are important for industrial assessment of sugarcane fermentation.