CAJ | 학술논문

为测定水产品中呋喃唑酮代谢物（AOZ ）的残留量，采用2-硝基苯甲醛进行衍生，乙酸乙酯提取AOZ。通过样品中残留的AOZ和酶标板上预包被的抗原竞争抗体，加入酶标二抗后与底物显色，测得样品中AOZ的残留量。测定了四组水产品中AOZ的残留量，测定浓度均小于1μg/kg ，结果为阴性。向空白样品中分别添加0．5μg/kg、1．5μg/kg和2μg/kg的AOZ标准品，回收率为90％、109％和101％。因此得出结论：采用酶联免疫法测定水产品中AOZ残留量操作简单，检测时间短，结果准确，是一种适合大批量水产样品筛选的检测方法。
위측정수산품중부남서동대사물（AOZ ）적잔류량，채용2-초기분갑철진행연생，을산을지제취AOZ。통과양품중잔류적AOZ화매표판상예포피적항원경쟁항체，가입매표이항후여저물현색，측득양품중AOZ적잔류량。측정료사조수산품중AOZ적잔류량，측정농도균소우1μg/kg ，결과위음성。향공백양품중분별첨가0．5μg/kg、1．5μg/kg화2μg/kg적AOZ표준품，회수솔위90％、109％화101％。인차득출결론：채용매련면역법측정수산품중AOZ잔류량조작간단，검측시간단，결과준학，시일충괄합대비량수산양품사선적검측방법。
Objective :ELISA for the analysis of furazolidone (AOZ) residues in aquatic products were determined .Method:Samples were derivatived with 2-nitrobenzaldehyde and extracted with ethylac-etate .The AOZ and microtiter board antigenic competition antibody ,chromogenic substrate with la-beled sencondary antibody ,determine the residues of AOZ samples .Results :When the samples were spiked with 0 .5 μg/kg ,1 .5 μg/kg and 2 μg/kg of AOZ ,the recoveries were 90% ,109% and 101%respectively by ELISA .Conclusion :ESLISA is a rapid and exact method for the screen of AOZ abun-dant samples in aquatic products .