广西医学
廣西醫學
엄서의학
GUANGXI MEDICAL JOURNAL
2014年
9期
1205-1208
,共4页
食管癌%磷脂酰乙醇胺结合蛋白4%畸胎瘤衍化生长因子%免疫组化法%蛋白质印迹法
食管癌%燐脂酰乙醇胺結閤蛋白4%畸胎瘤衍化生長因子%免疫組化法%蛋白質印跡法
식관암%린지선을순알결합단백4%기태류연화생장인자%면역조화법%단백질인적법
Esophageal carcinoma%Phosphatidylethanolamine binding protein 4%Teratocarcinoma derived growth factor/Cripto-1%Immunohistochemistry%Western Bolt
目的：探讨磷脂酰乙醇胺结合蛋白4（ PEBP4）、畸胎瘤衍化生长因子（ CR-1）在食管癌组织中表达的临床意义。方法选取食管癌组织（癌组织组）及正常食管黏膜组织（正常组）各30份，分别采用免疫组化法、蛋白质印迹法检测各组PEBP4、CR-1表达水平。结果癌组织组PEBP4表达阳性率为83．3％，显著高于正常组的20．0％（ P＜0．05）；有淋巴结转移及远处转移的癌灶PEBP4表达阳性率显著高于无淋巴结转移及无远处转移癌灶（P＜0．05）。癌组织组CR-1表达阳性率为86．7％，显著高于正常组的16．7％（P＜0．05）；有淋巴结转移及远处转移的癌灶CR-1表达阳性率显著高于无淋巴结转移及无远处转移癌灶（ P＜0．05）。癌组织组CR-1 mRNA及其蛋白相对表达水平均显著高于正常组（ P＜0．05）。结论食管癌组织中PEBP4、CR-1阳性表达率较高，对食管癌的早期诊断及预后判断有较高临床价值。
目的：探討燐脂酰乙醇胺結閤蛋白4（ PEBP4）、畸胎瘤衍化生長因子（ CR-1）在食管癌組織中錶達的臨床意義。方法選取食管癌組織（癌組織組）及正常食管黏膜組織（正常組）各30份，分彆採用免疫組化法、蛋白質印跡法檢測各組PEBP4、CR-1錶達水平。結果癌組織組PEBP4錶達暘性率為83．3％，顯著高于正常組的20．0％（ P＜0．05）；有淋巴結轉移及遠處轉移的癌竈PEBP4錶達暘性率顯著高于無淋巴結轉移及無遠處轉移癌竈（P＜0．05）。癌組織組CR-1錶達暘性率為86．7％，顯著高于正常組的16．7％（P＜0．05）；有淋巴結轉移及遠處轉移的癌竈CR-1錶達暘性率顯著高于無淋巴結轉移及無遠處轉移癌竈（ P＜0．05）。癌組織組CR-1 mRNA及其蛋白相對錶達水平均顯著高于正常組（ P＜0．05）。結論食管癌組織中PEBP4、CR-1暘性錶達率較高，對食管癌的早期診斷及預後判斷有較高臨床價值。
목적：탐토린지선을순알결합단백4（ PEBP4）、기태류연화생장인자（ CR-1）재식관암조직중표체적림상의의。방법선취식관암조직（암조직조）급정상식관점막조직（정상조）각30빈，분별채용면역조화법、단백질인적법검측각조PEBP4、CR-1표체수평。결과암조직조PEBP4표체양성솔위83．3％，현저고우정상조적20．0％（ P＜0．05）；유림파결전이급원처전이적암조PEBP4표체양성솔현저고우무림파결전이급무원처전이암조（P＜0．05）。암조직조CR-1표체양성솔위86．7％，현저고우정상조적16．7％（P＜0．05）；유림파결전이급원처전이적암조CR-1표체양성솔현저고우무림파결전이급무원처전이암조（ P＜0．05）。암조직조CR-1 mRNA급기단백상대표체수평균현저고우정상조（ P＜0．05）。결론식관암조직중PEBP4、CR-1양성표체솔교고，대식관암적조기진단급예후판단유교고림상개치。
Objective To explore the clinical significance of expressions of phosphatidylethanolamine binding protein 4(PEBP4),teratocarcinoma derived growth factor (Cripto-1,CR-1) in esophageal carcinoma tissues .Methods Thirty cases of esophageal cancer tissues and 30 case of normal esophageal mucosa tissues were selected ,respectively . The expressions of PEBP 4, CR-1 were detected by immunohistochemical technique and Western Blot , respectively . Results The positive rate of PEBP4 expression in the esophageal cancer group was 83.3%,significantly higher than that in the normal group (20.0%).The positive rate of PEBP4 expression in the lesion with lymph node metastasis and distant metastasis tumor was significantly higher than that without metastasis (P <0.05).The positive rate of CR-1 expression in the esophageal cancer group was 86.7%,significantly higher than that in the normal group(16.7%,P<0.05). The positive rate of CR-1 expression in the lesion with lymph node metastasis and distant metastasis tumor was significantly higher than that without metastasis(P<0.05).The relative expressions of CR-1 mRNA and protein in the esophageal cancer group were significantly higher than those in the normal group(P<0.05).Conclusion PEBP4 and CR-1 in esophageal cancer tissues have a higher positive expression rate ,which is valuable in the early diagnosis and prognosis of esophageal carcinoma .
