CAJ | 학술논문

目的：探讨CYP3 A5基因多态性与广西肝癌遗传易感性的关系。方法在广西肝癌高发区选取18个肝癌高发家族成员178例（高发组）及与之相对应的18个无肿瘤家族成员178例（对照组）为研究对象，用流行病学问卷调查方法收集两组相关资料，同时现场采集空腹外周静脉血10 ml，用多聚酶链-限制性片段长度多态性技术（PCR-RFLP）检测CYP3A5基因型。结果高发组GG基因型比例低于对照组（P＜0．01）， GA＋AA基因型比例高于对照组（P＜0．05）。高发组A等位基因比例高于对照组（P＜0．05）。结论 CYP3A5基因多态性与广西肝癌的遗传易感性密切相关。
목적：탐토CYP3 A5기인다태성여엄서간암유전역감성적관계。방법재엄서간암고발구선취18개간암고발가족성원178례（고발조）급여지상대응적18개무종류가족성원178례（대조조）위연구대상，용류행병학문권조사방법수집량조상관자료，동시현장채집공복외주정맥혈10 ml，용다취매련-한제성편단장도다태성기술（PCR-RFLP）검측CYP3A5기인형。결과고발조GG기인형비례저우대조조（P＜0．01）， GA＋AA기인형비례고우대조조（P＜0．05）。고발조A등위기인비례고우대조조（P＜0．05）。결론 CYP3A5기인다태성여엄서간암적유전역감성밀절상관。
Objective Objective To investigate the relationship between genetic polymorphism of CYP 3A5 and genetic susceptibility of hepatocellular carcinoma ( HCC ) in Guangxi .Methods One hundred and seventy-eight cases were collected from 18 HCC area of the high HCC incidence in Guangxi as high-risk group,and another 178 cases from 18 families with non-tumor history as control group .The relevant data of two groups were obtained through the epidemiological questionnaires .Concurrently , 10 ml fasting peripheral blood was collected .The genotype of CYP3A5 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results The genotype ratio of GG was lower but of GA +AA was higher in the high-risk group in contrast with those in the control group ( P<0 .05 ) .There was statistical difference in the A allele ratio between high-risk group and control group(P<0.05).Conclusion The gene polymorphism of CYP3A5 is closely associated with genetic susceptibility of HCC in Guangxi .