北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
9期
2-3,4
,共3页
薄层色谱法%高效液相色谱法%盐酸小檗碱%八味獐牙菜丸
薄層色譜法%高效液相色譜法%鹽痠小檗堿%八味獐牙菜汍
박층색보법%고효액상색보법%염산소벽감%팔미장아채환
TLC%HPLC%Berberine Hydrochloride%Bawei Zhangyacai Pill
目的：建立测定藏药八味獐牙菜丸中盐酸小檗碱含量的方法。方法：采用薄层色谱法和高效液相色谱法。结果：建立了八味獐牙菜丸中兔耳草和小檗皮的薄层色谱鉴别方法，并采用HPLC测定了该藏药制剂中小檗皮的有效成分盐酸小檗碱的含量，其回归方程为：y=4629102.46x-136034.70，r=0.9995，盐酸小檗碱的平均回收率为97.30%，RSD=1.57%（n=6）。结论：该方法测定快速、重现性好，可用于八味獐牙菜丸的质量控制。
目的：建立測定藏藥八味獐牙菜汍中鹽痠小檗堿含量的方法。方法：採用薄層色譜法和高效液相色譜法。結果：建立瞭八味獐牙菜汍中兔耳草和小檗皮的薄層色譜鑒彆方法，併採用HPLC測定瞭該藏藥製劑中小檗皮的有效成分鹽痠小檗堿的含量，其迴歸方程為：y=4629102.46x-136034.70，r=0.9995，鹽痠小檗堿的平均迴收率為97.30%，RSD=1.57%（n=6）。結論：該方法測定快速、重現性好，可用于八味獐牙菜汍的質量控製。
목적：건립측정장약팔미장아채환중염산소벽감함량적방법。방법：채용박층색보법화고효액상색보법。결과：건립료팔미장아채환중토이초화소벽피적박층색보감별방법，병채용HPLC측정료해장약제제중소벽피적유효성분염산소벽감적함량，기회귀방정위：y=4629102.46x-136034.70，r=0.9995，염산소벽감적평균회수솔위97.30%，RSD=1.57%（n=6）。결론：해방법측정쾌속、중현성호，가용우팔미장아채환적질량공제。
Objietive:To establish a TLC identification and an HPLC quantitative determination methods for Bawei Zhangyacai Pills. Methods: TLC and HPLC were used.Results:The TLC method could identify Lagotis glauca Gaertn and Berberis kansuensis Schneid , and HPLC method could quantitive determine Berberine Hydrochloride quantity in Bawei Zhangyacai Pills. The regression equation of standard curve was y=4629102.46x -136034.70,r =0.9995,the mean recovery was 97.30% with RSD of1.57% for the HPLC method. Conclusions:This method is simple, rapid and reliable with good reproducibility, and can be used to control the quality of Bawei Zhangyacai Pills.
