CAJ | 학술논문

目的：探讨免疫学标记在甲状腺细针穿吸细胞学诊断中应用的价值。方法应用免疫细胞化学技术，观察CD45 RO、Vimentin、HLA-DR、CK/Pan在60例桥本氏甲状腺炎（Hashimot′s thyroiditis，HT）和10例结节性甲状腺细胞的表达。结果（1）CD45RO、HLA-DR在其T淋巴细胞阳性率表达上HT高于结节性甲状腺肿，有显著差异（P＜0．05）；（2）甲状腺滤泡上皮细胞（TEC）的DR和Vimentin表达程度HT明显高于结节性甲状腺肿，有显著差异（P＜0．05）；（3）CK/Pan在不典型增生17例中，阳性表达10例（10/17），细胞学确诊甲状腺癌3例均阳性表达，在细胞无异型中1例强表达。结论细针穿刺加做CD45 RO、Vimentin、HLA-DR、CK/Pan有助于桥本甲状腺炎的分型及与其他甲状腺疾病的鉴别、判断疾病的发展程度，且能筛检出变异细胞为临床治疗诊断提供可靠依据。
목적：탐토면역학표기재갑상선세침천흡세포학진단중응용적개치。방법응용면역세포화학기술，관찰CD45 RO、Vimentin、HLA-DR、CK/Pan재60례교본씨갑상선염（Hashimot′s thyroiditis，HT）화10례결절성갑상선세포적표체。결과（1）CD45RO、HLA-DR재기T림파세포양성솔표체상HT고우결절성갑상선종，유현저차이（P＜0．05）；（2）갑상선려포상피세포（TEC）적DR화Vimentin표체정도HT명현고우결절성갑상선종，유현저차이（P＜0．05）；（3）CK/Pan재불전형증생17례중，양성표체10례（10/17），세포학학진갑상선암3례균양성표체，재세포무이형중1례강표체。결론세침천자가주CD45 RO、Vimentin、HLA-DR、CK/Pan유조우교본갑상선염적분형급여기타갑상선질병적감별、판단질병적발전정도，차능사검출변이세포위림상치료진단제공가고의거。
Objective Explortion the application of immunological markers in thyroid fine needle aspiration cytology.Methods Appli-cation of immunocytochemical technique,observes the expression of CD45RO,Vimentin,HLA-DR,CK/Pan in 60 cases of HT and 10cases of nodular Thyroid cells.Results (1)The T lympholytes positive expression of CD45RO,HLA-DR,HT is significantly higherthan normal nodular goiter(P <0.05).(2)The expression of DR and Vimentin in thyroid follicular epithelial,HT is significantly higherthan normal nodular goiter(P <0.05).(3)In 17 cases atypicalhyperplasia cases,the CK/Pan positive expression were 10 cases,cyto-logical diagnosis 3 thyroid carcinoma cases were positive expression,1 strong expression case in no abnormal cell morphology.Conclu-sions Cell puncture cooperate with CD45RO,Vimentin,HLA-DR,CK/Pan contribute to the types of thyroiditis,identification of otherthyroid diseases,determins disease severity,and screens variant cells could provide celiable basis for clinical diagnosis and treatment.