CAJ | 학술논문

目的：观察茶多酚对创伤性颅脑损伤大鼠的脑保护作用。方法通过可控性皮层打击器建立SD大鼠颅脑损伤模型。打击24 h后对大鼠进行神经功能缺损评分，测量干湿重以及静脉注射伊文士蓝的方法评价血脑屏障（BBB，Blood-brain barri-er）的通透性，采用蛋白免疫印迹的方法测量水肿相关蛋白aquaporin-4（AQP4）和matrix metalloproteinase-9（MMP-9）的表达。结果与模型组比较，给药组打击24 h后的神经功能缺损评分明显降低（P＜0．05），脑含水量和BBB开放程度明显减少（P＜0．05）。给药组大鼠的水肿相关蛋白AQP4、MMP-9的表达减少（P＜0．05）。结论茶多酚具有脑保护作用，其机制可能与减轻创伤后脑水肿程度，减少AQP4、MMP-9表达有关。
목적：관찰다다분대창상성로뇌손상대서적뇌보호작용。방법통과가공성피층타격기건립SD대서로뇌손상모형。타격24 h후대대서진행신경공능결손평분，측량간습중이급정맥주사이문사람적방법평개혈뇌병장（BBB，Blood-brain barri-er）적통투성，채용단백면역인적적방법측량수종상관단백aquaporin-4（AQP4）화matrix metalloproteinase-9（MMP-9）적표체。결과여모형조비교，급약조타격24 h후적신경공능결손평분명현강저（P＜0．05），뇌함수량화BBB개방정도명현감소（P＜0．05）。급약조대서적수종상관단백AQP4、MMP-9적표체감소（P＜0．05）。결론다다분구유뇌보호작용，기궤제가능여감경창상후뇌수종정도，감소AQP4、MMP-9표체유관。
Objective To investigate the protective effect of tea polyphenols on traumatic brain injury.Methods The models were es-tablished by controlled cortical impact,24 hours after the strike,Neurological Severity Scores and brain water content were observed,theinjury to the blood-brain barrier was also observed by the content of Evan’s blue (EB)in the cerebral tissue,moreover,the expresstionof aquaporin-4 (AQP4)and matrix metalloproteinase-9 (MMP-9)were measured by western blot.Results Tea polyphenols improvedNeurological Severity Scores (P <0.05),alleviated brain water content and the disruption of Blood-brain barrier (BBB)(P <0.05),as well as reduced the expression of AQP4 and MMP-9 (P <0.05).Conclustion Tea Polyphenols played a role in protecting thebrain after traumatic brain injury,The potential mechanism perhaps is reducing of the extent of brain edema by inhibition of the expres-sion of AQP4 and MMP-9.