CAJ | 학술논문

目的：观察卵巢去势1个月对大鼠切牙牙髓组织中成牙、成骨指标的影响。方法：将10只成年雌性SD大鼠（8周龄）随机分为假手术组及卵巢去势组，每组各5只；在全麻下，将去势组大鼠双侧卵巢摘除，假手术组行相同切口，保留卵巢；1个月后，处死大鼠，分离牙髓组织，通过实时定量RT-PCR及Westernblot方法检测两组大鼠牙髓组织中成牙、成骨相关指标的表达。结果：去势组牙髓组织中，Runt相关转录因子2（Runt-relatedtranscriptionfactor2，RUNX2）、骨细胞特异性转录因子（Os-terix，OSX）、骨钙素（Osteocalcin，OCN）、牙本质涎蛋白（dentinsialoprotein，DSP）、牙本质涎磷蛋白（dentinsialophosphoprotein，DSPP）的基因和/或蛋白表达等成牙及成骨指标均较假手术组牙髓低，而碱性磷酸酶（alkalinephosphatase，ALP）的表达无明显改变。结论：卵巢去势1个月可以明显降低大鼠切牙牙髓组织中成牙、成骨指标的表达，提示卵巢去势下调了牙髓细胞的成牙及成骨能力。
목적：관찰란소거세1개월대대서절아아수조직중성아、성골지표적영향。방법：장10지성년자성SD대서（8주령）수궤분위가수술조급란소거세조，매조각5지；재전마하，장거세조대서쌍측란소적제，가수술조행상동절구，보류란소；1개월후，처사대서，분리아수조직，통과실시정량RT-PCR급Westernblot방법검측량조대서아수조직중성아、성골상관지표적표체。결과：거세조아수조직중，Runt상관전록인자2（Runt-relatedtranscriptionfactor2，RUNX2）、골세포특이성전록인자（Os-terix，OSX）、골개소（Osteocalcin，OCN）、아본질연단백（dentinsialoprotein，DSP）、아본질연린단백（dentinsialophosphoprotein，DSPP）적기인화/혹단백표체등성아급성골지표균교가수술조아수저，이감성린산매（alkalinephosphatase，ALP）적표체무명현개변。결론：란소거세1개월가이명현강저대서절아아수조직중성아、성골지표적표체，제시란소거세하조료아수세포적성아급성골능력。
Objective:To investigate the effects of ovariectomy on the expression of odonto /osteogenic markers in rat incisor pulps.Methods:Ten 8-week-old female SD rats were randomly divided into 2 groups:Ovariectomized(OVX) group (n=5) and Sham-opera-tion(Sham) group (n=5).All rats were anesthetized and both ovaries of OVX-rats were gently removed.Sham-operation rats were treated with the same incisions to expose the ovaries but without any hurt to them.One month after the operation, the incisor pulps were respectively isolated.Real-time RT-PCR and Western blot were performed to evaluate the expression of odonto /osteogenic markers in the two groups.Results:The expression of odonto/osteogenic markers (i.e., RUNX2/RUNX2, OSX/OSX, OCN/OCN, and DSPP/DSP) in OVX group was significantly weaker than that in Sham group.However,the expression of ALP was not significantly changed.Conclusions:Ovariectomy can downregulate the expression of odonto/osteogenic markers in dental pulps, indicating that ovariectomy decreased the odonto/osteogenic capacity of dental pulp cells.