CAJ | 학술논문

目的：研究Rap1b与斑马鱼颅面部发育的关系。方法：利用模式动物斑马鱼，取由神经嵴发育而来的颅面部组织做实时定量RT-PCR，观察不同时期Rap1b的mRNA表达情况。再通过Rap1b整体原位杂交观察其在斑马鱼发育过程中时间和空间的表达情况，并与神经嵴特异性标志基因Crestin原位杂交表达情况在时间和空间上对比。结果：实时定量RT-PCR结果显示，Rap1b在神经嵴形成、迁移、分化过程中在颅面部均有表达。原位杂交结果显示，Rap1b与Crestin在斑马鱼中的表达在时间和空间上有重叠。结论：Rap1b可能通过调节神经嵴发育参与颅面部发育。
목적：연구Rap1b여반마어로면부발육적관계。방법：이용모식동물반마어，취유신경척발육이래적로면부조직주실시정량RT-PCR，관찰불동시기Rap1b적mRNA표체정황。재통과Rap1b정체원위잡교관찰기재반마어발육과정중시간화공간적표체정황，병여신경척특이성표지기인Crestin원위잡교표체정황재시간화공간상대비。결과：실시정량RT-PCR결과현시，Rap1b재신경척형성、천이、분화과정중재로면부균유표체。원위잡교결과현시，Rap1b여Crestin재반마어중적표체재시간화공간상유중첩。결론：Rap1b가능통과조절신경척발육삼여로면부발육。
Objective:To find the relationship between Rap1b and the zebrafish craniolfacial development.Methods:The expression of Rap1b in the head of zebrafish,which was derived from neural crest by real-time RT-PCR,was detected.Then compare the expression of Rap1b and Crestin was compared in the process of zebrafish development with in situ hybridization.Results:The results of real-time RT-PCR indicated that Rap1b was expressed in the craniofacial of zebrafish during the neural crest induction , migration and derivation.The results of in situ hybridization indicated that the expression of Rap1b and Crestin were partial overlapped in the dimention of time and space of zebrafish develpment.Conclusions:Rap1b may influence the development of zebrafish craniofacial part by regulating the development of neural crest.