CAJ | 학술논문

目的探讨传统中药人参皂苷Rg1对血管紧张素Ⅱ（ AngⅡ）所诱发心肌肥大的影响。方法采用酶消化法分离新生大鼠心室肌细胞，培养心肌细胞随机分为3组：正常对照组、Ang Ⅱ组、AngⅡ＋Rg1组。在接受药物处理24h后，检测心肌细胞总蛋白含量，β-MHC、TNF-α和IL-1β的mRNA表达量。结果 Ang Ⅱ处理导致培养心肌细胞的总蛋白含量以及β-MHC mRNA表达量显著增加，提示心肌肥大的发生；人参皂苷Rg1显著抑制了Ang Ⅱ的促心肌细胞肥大作用。同时，AngⅡ增加心肌细胞对TNF-α和IL -1βmRNA表达的效应也被人参皂苷Rg1所抑制。结论人参皂苷Rg1抑制了 AngⅡ诱导的心肌细胞肥大以及心肌细胞对炎性介质TNF-α、IL-1β的释放，这可能是人参皂苷Rg1对心脏疾病具有保护作用的细胞学基础。
목적탐토전통중약인삼조감Rg1대혈관긴장소Ⅱ（ AngⅡ）소유발심기비대적영향。방법채용매소화법분리신생대서심실기세포，배양심기세포수궤분위3조：정상대조조、Ang Ⅱ조、AngⅡ＋Rg1조。재접수약물처리24h후，검측심기세포총단백함량，β-MHC、TNF-α화IL-1β적mRNA표체량。결과 Ang Ⅱ처리도치배양심기세포적총단백함량이급β-MHC mRNA표체량현저증가，제시심기비대적발생；인삼조감Rg1현저억제료Ang Ⅱ적촉심기세포비대작용。동시，AngⅡ증가심기세포대TNF-α화IL -1βmRNA표체적효응야피인삼조감Rg1소억제。결론인삼조감Rg1억제료 AngⅡ유도적심기세포비대이급심기세포대염성개질TNF-α、IL-1β적석방，저가능시인삼조감Rg1대심장질병구유보호작용적세포학기출。
Objective To determine the effect of ginsenoside Rg 1 on angiotensinⅡ ( AngⅡ)-induced myocardial hypertrophy .Methods Cardiomyocytes were isolated from 1~3 days old Sprague-Dawley rats and then randomly divided into 3 groups:normal control,Ang Ⅱgroup,Ang Ⅱ+Rg1 group.After 24h treatment, cardiomyocytes were collected to determine the total protein content by Lowry’ s method, and β-myosin heavy chain (β-MHC) ,TNF-α,and IL-1βmRNA expression was detected by Real-time PCR.Results Ang Ⅱ in-duced cardiomyocyte hypertrophy as evidenced by increased total protein content and enhanced expression of β-MHC mRNA.Ginsenoside Rg1 significantly inhibited these hypertrophic responses , suppressed AngⅡ-in-duced increase of TNF-αand IL-1βmRNA expression in cardiomyocytes .Conclusion Ginsenoside Rg1 can in-hibite cardiomyocyte hypertrophy induced by AngⅡ-and increase the release of inflammatory mediators such as TNF-αand IL-1β,which may be the cellular basis for its protective role in cardiac diseases .