CAJ | 학술논문

为了在毕赤酵母Pichia pastoris表达系统中表达猪源Cecropin P1并分析其生物活性，根据Cecropin P1的氨基酸序列和毕赤酵母的密码子偏嗜性设计引物，通过SOEing法合成全基因片段载入到PpicZαA质粒中，酶切线性化重组质粒PpicZαA-cecropin P1后电穿孔导入毕赤酵母X-33中进行整合，经Zection筛选和PCR检测获得高拷贝的转化子。发酵表达后将表达产物经加热预处理和Sephadex G-25层析及冻干浓缩后，Tricine-SDS-PAGE显示明亮单一条带。采用琼脂扩散法对纯化后的抗菌肽的热稳定性、酸碱稳定性、耐胰酶和胃蛋白酶稳定性进行了生物活性分析，结果表明Cecropin P1具有较强的热稳定性、酸稳定性及胃蛋白酶稳定性，但是在碱性环境下和胰酶作用下抑菌活性明显下降。
위료재필적효모Pichia pastoris표체계통중표체저원Cecropin P1병분석기생물활성，근거Cecropin P1적안기산서렬화필적효모적밀마자편기성설계인물，통과SOEing법합성전기인편단재입도PpicZαA질립중，매절선성화중조질립PpicZαA-cecropin P1후전천공도입필적효모X-33중진행정합，경Zection사선화PCR검측획득고고패적전화자。발효표체후장표체산물경가열예처리화Sephadex G-25층석급동간농축후，Tricine-SDS-PAGE현시명량단일조대。채용경지확산법대순화후적항균태적열은정성、산감은정성、내이매화위단백매은정성진행료생물활성분석，결과표명Cecropin P1구유교강적열은정성、산은정성급위단백매은정성，단시재감성배경하화이매작용하억균활성명현하강。
To express the porcine antibacterial peptide Cecropin P 1 in Pichia pastoris and analyze the bio-logical activity of the expressed product , Cecropin P1 gene was designed and synthesized to include the partiality codons of P.pastoris based on the gene sequences encoding swine antibacterial Cecropin P 1.The linearized recombinant was shocked into P.pastoris X-33 by electroporation , and the high copy transfor-mants were screened with Zection and PCR .After being pured , Tricine -SDS-PAGE showed a single bright band .Agar diffusion method was used to analyze the biological activity of the purified antimicrobial peptides under the thermal , different pH , trypsin and pepsin .The results indicated that the cecropin P 1 had a strong thermal stability , acid stability and pepsin stability .However , in alkaline environment and pancreatin , the antibacterial activity of cecropin P 1 decreased significantly .