CAJ | 학술논문

采用2种不同的逆转录病毒载体系统（pMX和pMSCV），构建携带绿色荧光蛋白（EGFP）基因的重组载体，探索能高效感染水牛胎儿成纤维细胞（ BFFs）的病毒系统和感染方法。结果发现，2种逆转录病毒系统包装出的重组病毒滴度都能达到106，将pMX病毒系统生产的重组病毒经超速离心浓缩后，滴度可达到107；2种重组病毒都能感染BFFs，但pMX系统比pMSCV系统更能有效地感染BFFs；通过对病毒的感染方式进行比较，发现使用新鲜病毒连续感染BFFs 2次（每次间隔12 h），或者经过超速离心浓缩后的病毒感染BFFs 1次，可以显著提高感染效率。
채용2충불동적역전록병독재체계통（pMX화pMSCV），구건휴대록색형광단백（EGFP）기인적중조재체，탐색능고효감염수우태인성섬유세포（ BFFs）적병독계통화감염방법。결과발현，2충역전록병독계통포장출적중조병독적도도능체도106，장pMX병독계통생산적중조병독경초속리심농축후，적도가체도107；2충중조병독도능감염BFFs，단pMX계통비pMSCV계통경능유효지감염BFFs；통과대병독적감염방식진행비교，발현사용신선병독련속감염BFFs 2차（매차간격12 h），혹자경과초속리심농축후적병독감염BFFs 1차，가이현저제고감염효솔。
After two retroviral systems with enhanced green fluorescent protein ( EGFP) report gene were constructed in this study , the retroviral systems and the infection method suitable for buffalo fetal fibro-blasts (BFFs) were investigated.The results revealed that the retrovirus titer of the two systems was 106, and the titer of the pMX system could be up to 10 7 after ultracentrifugation concentration .The infection efficiency of pMX retroviral system was higher than that of the pMSCV retroviral system .Using the fresh retrovirus infected BFFs twice (12 h interval) or ultracentrifugal retrovirus infected BFFs once could im-prove the infection efficiency .