华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2013年
4期
450-457
,共8页
陈杰%杨静%陈建军%赵伟才%蔡一霞%邓世媛%王维
陳傑%楊靜%陳建軍%趙偉纔%蔡一霞%鄧世媛%王維
진걸%양정%진건군%조위재%채일하%산세원%왕유
烤烟%种质资源%形态学标记%SSR标记%遗传多样性%聚类分析
烤煙%種質資源%形態學標記%SSR標記%遺傳多樣性%聚類分析
고연%충질자원%형태학표기%SSR표기%유전다양성%취류분석
flue-cured tobacco%germplasm resource%morphological marker%SSR markers%genetic di-versity%cluster analysis
田间调查了69份烤烟品种形态学性状,并进行各品种间的遗传距离分析;对69份烤烟种质资源进行SSR分析,估算遗传相似系数;结合这2种方法对69份烤烟资源进行遗传多样性研究.结果表明,69份烤烟种质材料间的遗传距离在0.32~7.83之间,平均为2.80,其遗传相似系数在0.30~0.96之间,平均为0.70.聚类分析结果为,供试品种大致分为国外优质种质及其衍生的后代、国内外地方品种、早花类型品种、晚花类型品种等几个类群.结合形态学标记和分子标记对烤烟种质资源进行遗传多样性评价,揭示了烤烟种间的遗传多样性与亲缘关系.
田間調查瞭69份烤煙品種形態學性狀,併進行各品種間的遺傳距離分析;對69份烤煙種質資源進行SSR分析,估算遺傳相似繫數;結閤這2種方法對69份烤煙資源進行遺傳多樣性研究.結果錶明,69份烤煙種質材料間的遺傳距離在0.32~7.83之間,平均為2.80,其遺傳相似繫數在0.30~0.96之間,平均為0.70.聚類分析結果為,供試品種大緻分為國外優質種質及其衍生的後代、國內外地方品種、早花類型品種、晚花類型品種等幾箇類群.結閤形態學標記和分子標記對烤煙種質資源進行遺傳多樣性評價,揭示瞭烤煙種間的遺傳多樣性與親緣關繫.
전간조사료69빈고연품충형태학성상,병진행각품충간적유전거리분석;대69빈고연충질자원진행SSR분석,고산유전상사계수;결합저2충방법대69빈고연자원진행유전다양성연구.결과표명,69빈고연충질재료간적유전거리재0.32~7.83지간,평균위2.80,기유전상사계수재0.30~0.96지간,평균위0.70.취류분석결과위,공시품충대치분위국외우질충질급기연생적후대、국내외지방품충、조화류형품충、만화류형품충등궤개류군.결합형태학표기화분자표기대고연충질자원진행유전다양성평개,게시료고연충간적유전다양성여친연관계.
The genetic distance of sixty-nine flue-cured tobacco germplasm resources were analyzed by the field performance characters and SSR markers .The genetic distance was 0.32-7.83 among 69 flue-cured tobacco germplasm varieties , with an average of 2.80.The genetic similarity index was 0.30 -0.96 among 69 flue-cured tobacco varieties , with an average of 0.70 .Cluster analysis based on the ma-trix of genetic distance and genetic similarity index , respectively, was performed.Clustering results showed that the tested tobacco varieties could be divided into foreign varieties , domestic varieties , early-flowering type , late-flowering type etc ..The combination of morphological markers and molecular mark-ers to evaluate the genetic diversity of flue-cured tobacco germplasm resources may reveal the interspecific genetic diversity and genetic relationship of tobacco , providing the theory basis for parental selection in cross breeding of tobacco genetic breeding .