CAJ | 학술논문

目的探索Sertoli细胞（SCs）对骨髓间充质干细胞（BMSCs）成脂、成骨和成神经诱导分化的影响。方法密度梯度离心法分离BMSCs，二步酶消化法分离SCs；对第3、4代BMSCs进行成脂、成骨及成神经诱导，并与SCs共培养，观察其形态学变化。结果油红O染色显示BMSCs成脂诱导后，胞浆出现折光性强的脂滴，加入SCs后，脂滴明显减少，染色不明显；茜素红S染色显示BMSCs成骨诱导后可显示钙结节，加入SCs后，钙结节更多，结节内红染更明显；成神经诱导在加入SCs前后没有明显差异。结论 SCs可促进BMSCs成骨分化，而抑制其成脂分化，对成神经分化则没有明显影响。
목적탐색Sertoli세포（SCs）대골수간충질간세포（BMSCs）성지、성골화성신경유도분화적영향。방법밀도제도리심법분리BMSCs，이보매소화법분리SCs；대제3、4대BMSCs진행성지、성골급성신경유도，병여SCs공배양，관찰기형태학변화。결과유홍O염색현시BMSCs성지유도후，포장출현절광성강적지적，가입SCs후，지적명현감소，염색불명현；천소홍S염색현시BMSCs성골유도후가현시개결절，가입SCs후，개결절경다，결절내홍염경명현；성신경유도재가입SCs전후몰유명현차이。결론 SCs가촉진BMSCs성골분화，이억제기성지분화，대성신경분화칙몰유명현영향。
Objective To explore the effects of Sertoli cells on the induced adipogenic, osteogenic and neuroblastic differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods BMSCs were isolated and purified with the density gradient centrifugation method, Sertoli cells were isolated with two-step enzyme digestion;The adipogenic, osteogenic and neuroblastic differentiation of P3/P4 BMSCs were induced. Sertoli cells were co-cultured with BMSCs and then the morphological change of BMSCs were observed. Results After adipogenic induction, the lipid droplets that have strong refraction in the cytoplasm were observed by oil red O staining. The intracellular lipid droplets shrunk and the orange stained lipid droplets were hard to observe after adding Sertoli cells; After osteogenic induction, calcium nodules were shown by alizarin red S staining. More clearer calcium nodules were observed and the red-staining was stronger after adding Sertoli cells. No morphological change was observed on BMSCs before and after adding Sertoli cells. Conclusion Sertoli cells could promote BMSCs’ osteogenic differentiation, inhibit their adipogenic differentiation and have no effect on their neuroblastic differentiation.