CAJ | 학술논문

目的将含hTERT基因的重组慢病毒液感染大鼠骨髓间充质干细胞（Bone marrow mesenchymal stem cells， BMSCs），鉴定其生物学特性。方法分别将含hTERT基因重组慢病毒液、空病毒液感染大鼠BMSCs，实验分为MSC-hTERT组、MSC-GFP组和MSC组（未感染组）。体外培养扩增后，通过定量PCR对各组目的基因表达进行检测。观察MSC组及MSC-hTERT组的体外传代次数及细胞形态变化，分析细胞周期。流式细胞仪检测MSC-hTERT组细胞标志物，评估其成骨、成脂分化能力。结果 MSC-hTERT组目的基因mRNA表达均高于另两组，体外培养过程中MSC-hTERT组传代次数和增殖指数高于MSC组，且具备多向分化潜能。结论转染hTERT的BMSCs具备BMSCs同样的生物学特性，且细胞增殖能力加强，可为组织工程研究以及细胞移植治疗提供可靠的种子细胞。
목적장함hTERT기인적중조만병독액감염대서골수간충질간세포（Bone marrow mesenchymal stem cells， BMSCs），감정기생물학특성。방법분별장함hTERT기인중조만병독액、공병독액감염대서BMSCs，실험분위MSC-hTERT조、MSC-GFP조화MSC조（미감염조）。체외배양확증후，통과정량PCR대각조목적기인표체진행검측。관찰MSC조급MSC-hTERT조적체외전대차수급세포형태변화，분석세포주기。류식세포의검측MSC-hTERT조세포표지물，평고기성골、성지분화능력。결과 MSC-hTERT조목적기인mRNA표체균고우령량조，체외배양과정중MSC-hTERT조전대차수화증식지수고우MSC조，차구비다향분화잠능。결론전염hTERT적BMSCs구비BMSCs동양적생물학특성，차세포증식능력가강，가위조직공정연구이급세포이식치료제공가고적충자세포。
Objective To identify the biological characteristics after infecting the rat BMSCs with recombinant virus supernatant containing hTERT gene. Methods BMSCs were infected with hTERT recombinant virus supernatant (MSC-hTERT group) and empty virus supernatant (MSC-GFP group), the uninfected group (MSC group) was also set up. The expression of target gene was detected by realtime PCR. Number of passages, cell morphology and cell cycle in MSC group and MSC-hTERT group were observed and analyzed. Cell markers of cells in MSC-hTERT group were detected and the potential of differentiation was also evaluated. Results The expression of hTERT was higher in MSC-hTERT group than in the other two groups. Number of passages and proliferation index in MSC-hTERT group were higher than in MSC group. The ability of multi-lineage differentiation potential of cells in MSC-hTERT group was maintained. Conclusion MSCs transfected by hTERT have the same biological characteristics as primary BMSCs and the ability of cell proliferation was strengthened. More cells could be reserved for tissue engineering and cell transplantation therapy.