CAJ | 학술논문

目的探索以骨髓间充质干细胞（BMSCs）为种子细胞，在体外构建具有完整内侧半月板形态的软骨样组织的方法。方法运用模具制备内侧半月板形的PGA/PLA支架。抽取犬骨髓，分离培养BMSCs，将其接种于支架材料上，5 d后使用软骨诱导液培养。体外培养6周后，行大体观察、组织学检测及生物力学检测。结果细胞材料复合物能够较好地维持半月板三维立体结构，形成了表面光滑、触之有弹性的瓷白色软骨样组织。 HE染色可见典型的软骨陷窝出现，说明成熟软骨组织的形成。Safranine O染色证实有蛋白聚糖基质产生。生物力学检测显示，新生组织弹性模量达正常半月板组织的12.7%。结论 BMSCs通过体外诱导，可在体外分化为较成熟的软骨组织，并构建出组织工程化半月板。
목적탐색이골수간충질간세포（BMSCs）위충자세포，재체외구건구유완정내측반월판형태적연골양조직적방법。방법운용모구제비내측반월판형적PGA/PLA지가。추취견골수，분리배양BMSCs，장기접충우지가재료상，5 d후사용연골유도액배양。체외배양6주후，행대체관찰、조직학검측급생물역학검측。결과세포재료복합물능구교호지유지반월판삼유입체결구，형성료표면광활、촉지유탄성적자백색연골양조직。 HE염색가견전형적연골함와출현，설명성숙연골조직적형성。Safranine O염색증실유단백취당기질산생。생물역학검측현시，신생조직탄성모량체정상반월판조직적12.7%。결론 BMSCs통과체외유도，가재체외분화위교성숙적연골조직，병구건출조직공정화반월판。
Objective To explore the techniques of constructing tissue engineered meniscus with bone marrow mesenchymal stem cells (BMSCs) in dog model in vitro. Methods Biodegradable scaffolds in shape of meniscus were fabricated with polyglycolic acid (PGA) and polylactic acid (PLA) by mold. BMSCs were harvested from bone marrow of dogs and cultured in vitro. Then the expanded cells were seeded onto the scaffolds, and transferred to chondrogenic medium after 5 days. Gross observation, histological observation and biomechanical test were carried out after cultured for 6 weeks. Results The constructs cultured in vitro for 6 weeks maintained three-dimensional structure of meniscus approximately, and formed smooth white cartilage-like tissue with elasticity. HE staining showed typical mature lacuna, suggesting the formation of mature cartilage tissue. Safranin'O staining showed abundant proteoglycan. Biomechanical analyses indicated that Young's modulus of constructs can reach 13.2% of native meniscus. Conclusion BMSCs can differentiate into heterogeneous cartilage-like tissue successfully through cultivation of inducing system, and construct tissue engineered meniscus in vitro.