CAJ | 학술논문

以葡萄球菌肠毒素B （S EB ）蛋白为模板分子，以聚苯乙烯微球为基质，采用表面分子印迹法制备了S EB分子印迹聚合物．利用平衡吸附试验分析了S EB聚合物对目标蛋白的吸附能力及对类似底物的选择性；分析了该聚合物的吸附动力学，并利用扫描电镜观察了其形貌特征和颗粒尺寸．结果表明，经Scatchard模型分析求得的标题聚合物的最大表观结合量 Qmax为3．23 mg/g ；所制备的SEB分子印迹聚合物呈微球形，粒径约为1～2μm ，对S EB蛋白具有较好的吸附性和特异选择性．
이포도구균장독소B （S EB ）단백위모판분자，이취분을희미구위기질，채용표면분자인적법제비료S EB분자인적취합물．이용평형흡부시험분석료S EB취합물대목표단백적흡부능력급대유사저물적선택성；분석료해취합물적흡부동역학，병이용소묘전경관찰료기형모특정화과립척촌．결과표명，경Scatchard모형분석구득적표제취합물적최대표관결합량 Qmax위3．23 mg/g ；소제비적SEB분자인적취합물정미구형，립경약위1～2μm ，대S EB단백구유교호적흡부성화특이선택성．
A molecularly imprinted polymer (M IP ) possessing specific molecule recognition ability for staphylococcus enterotoxin B (SEB) protein was synthesized by molecular imprinting technique with SEB as the template molecule and polystyrene microsphere as the substrate . Equilibrium binding experiments were used to investigate the binding ability of as-synthesized MIP beads for template molecule and their selectivity for several similar substrates .The ad-sorption dynamics of SEB-imprinted polymer beads was investigated ;and their morphology and size were examined with a scanning electron microscope .Results indicate that the apparent maximum adsorption capacity (Qmax ) of the title polymer beads ,calculated based on Scatchard model analysis ,is 3 .23 mg/g .Besides ,as-obtained SEB-MIP beads appear as microspheres with a diameter of approximately 1-2 μm and they exhibit good adsorption capacity and selec-tive recognition ability for SEB protein .