化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2013年
5期
64-65
,共2页
酶抑制法%农药残留快速测定仪%校准%吸光度%灵敏度
酶抑製法%農藥殘留快速測定儀%校準%吸光度%靈敏度
매억제법%농약잔류쾌속측정의%교준%흡광도%령민도
enzyme inhibition method%instrument for rapaid determination of pesticide residue%calibration%absorbency%sensitivity
用农药残留测定仪在波长412 nm处测定农药吸光度的变化值，根据酶抑制法计算抑制率，抑制率与农药浓度呈正相关。用农药标准物质从低于规定的农药检测下限开始配制一系列标准溶液对仪器进行校准，在抑制率为50%处的浓度即为仪器的灵敏度。对影响校准结果的因素进行分析，该方法校准结果的相对扩展不确定度为4%。
用農藥殘留測定儀在波長412 nm處測定農藥吸光度的變化值，根據酶抑製法計算抑製率，抑製率與農藥濃度呈正相關。用農藥標準物質從低于規定的農藥檢測下限開始配製一繫列標準溶液對儀器進行校準，在抑製率為50%處的濃度即為儀器的靈敏度。對影響校準結果的因素進行分析，該方法校準結果的相對擴展不確定度為4%。
용농약잔류측정의재파장412 nm처측정농약흡광도적변화치，근거매억제법계산억제솔，억제솔여농약농도정정상관。용농약표준물질종저우규정적농약검측하한개시배제일계렬표준용액대의기진행교준，재억제솔위50%처적농도즉위의기적령민도。대영향교준결과적인소진행분석，해방법교준결과적상대확전불학정도위4%。
According to the principle of enzyme inhibition method, the pesticide residues were determined at wavelength of 412 nm and the absorbance of the instrument value was used to calculate the inhibition rate, which was positively correlated with concentration. With a certificate of pesticide standard substances to configure a series solution for the calibration of the instrument from the concentration below the detection limit in pesticide regulations, the inhibitory rate of concentration 50%was the sensitivity of the instrument, and the influence of calibration results were analyzed. The relative expanded uncertaiinty of calibration results was 4%.
