CAJ | 학술논문

建立了茚三酮衍生高效毛细管电泳法测定纯奶中甘氨酸含量的方法，该法可选择性测定甘氨酸含量。使用氯化镁为蛋白沉淀剂，沉淀效果较好，研究了甘氨酸-茚三酮聚合物衍生条件，确定最佳实验条件为：未涂层弹性石英毛细管柱（60 cm×75μm，有效长度49 cm），分离缓冲溶液为3.5 mmol磷酸二氢钾-8.2 mmol磷酸氢二钠（pH 6.8），检测波长为570 nm，电泳电压为25 kV，进样压力为25 kPa，进样时间为3 s，电泳温度为室温。甘氨酸的线性范围为2.00～200.00μg/mL，检出限为0.14μg/mL，线性相关系数不小于0.999。甘氨酸的加标回收率为88.7%～107.2%，测定结果的相对标准偏差为2.9%～4.2%(n=6)。该法简便、快速、准确，可用于测定纯奶中甘氨酸含量。
건립료인삼동연생고효모세관전영법측정순내중감안산함량적방법，해법가선택성측정감안산함량。사용록화미위단백침정제，침정효과교호，연구료감안산-인삼동취합물연생조건，학정최가실험조건위：미도층탄성석영모세관주（60 cm×75μm，유효장도49 cm），분리완충용액위3.5 mmol린산이경갑-8.2 mmol린산경이납（pH 6.8），검측파장위570 nm，전영전압위25 kV，진양압력위25 kPa，진양시간위3 s，전영온도위실온。감안산적선성범위위2.00～200.00μg/mL，검출한위0.14μg/mL，선성상관계수불소우0.999。감안산적가표회수솔위88.7%～107.2%，측정결과적상대표준편차위2.9%～4.2%(n=6)。해법간편、쾌속、준학，가용우측정순내중감안산함량。
A method for the determination of glycine content in milk by high performance capillary electrophoresis with ninhydrin derivatization was established. The method was used for the selective determination of the glycine content. The derivatization of glycine-ninhydrin polymer was investigated,and the magnesium chlorate was applied to precipitating protein. The optimal experimental conditions were studied. An uncoated capillary (60 cm×75 μm,49 cm effective length) was used with running buffer solution of 3.5 mmol potassium dihydrogen phosphate-8.2 mmol disodium hydrogen phosphate. The detection wavelength was set at 750 nm and the run voltage was 25 kV. The injection time was 3 s at 25 kPa. The experiment was operated at room temperature. The results showed that the linear range for glycine was 2.00-200.00 mg/L,and the detection limit was 0.14μg/mL,the linear correlation coefficient of glycine was more than 0.999. The average recovery of glycine was between 88.7%-107.2%with the relative standard deviations of 2.9%-4.2%(n=6). This method is simple,rapid,and accurate,and it is suitable for the determination of the glycine content in milk.