CAJ | 학술논문

采用紫外衍生剂对甲氧基苯磺酰氯（MOBS-Cl）对加巴喷丁进行衍生反应，建立了一种柱前衍生测定加巴喷丁的新方法。最佳衍生化条件：对甲氧基苯磺酰氯与加巴喷丁的质量比大于7.5∶1，于50℃水浴中反应30 min。以0.05 mol/L Na2HPO4缓冲液（pH 10.0）-甲醇混合液为流动相，分析柱为反相ODS柱（150 mm×4.6 mm，5μm），柱温为室温，流速为0.8 mL/min，检测波长为240 nm。加巴喷丁溶液质量浓度在1～100 mg/L范围内与色谱峰面积线性关系良好，线性方程为A=24.5667c+12.3025，相关系数r=0.9999，测定结果的相对标准偏差(RSD)为0.56%，加巴喷丁的检测下限为0.017μg/g。
채용자외연생제대갑양기분광선록（MOBS-Cl）대가파분정진행연생반응，건립료일충주전연생측정가파분정적신방법。최가연생화조건：대갑양기분광선록여가파분정적질량비대우7.5∶1，우50℃수욕중반응30 min。이0.05 mol/L Na2HPO4완충액（pH 10.0）-갑순혼합액위류동상，분석주위반상ODS주（150 mm×4.6 mm，5μm），주온위실온，류속위0.8 mL/min，검측파장위240 nm。가파분정용액질량농도재1～100 mg/L범위내여색보봉면적선성관계량호，선성방정위A=24.5667c+12.3025，상관계수r=0.9999，측정결과적상대표준편차(RSD)위0.56%，가파분정적검측하한위0.017μg/g。
A new UV-derived agents 4-Methoxybenzenesulfonyl chloride(MOBS-Cl)of gabapentin was used to establish a method to determine gabapentin by a pre-column derivatization. The best conditions were:the mass ratio of 4-Methoxybenzenesulfonyl chloride and gabapentin was greater than 7.5∶1, the reaction lasted for 30 min at 50℃(water bath). 0.05 mol/L Na2HPO4 buffer(pH 10.0)-methanol was as the mobile phase, reversed-phase ODS column(150 mm×4.6 mm,5μm)as the analytical column, column temperature at room temperature with the flow rate of 0.8 mL/min and detection wavelength of 240 nm,the chromatography peak area was linear relationship with the mass concent in the range of 1-100 mg/L,the linear equation was A=24.566 7c(mg/L)+12.302 5,r=0.999 9,the relative standard deviation was 0.56%, the detection limit was 0.017μg/g.