CAJ | 학술논문

建立了一种高效凝胶排阻色谱快速测定牛血清白蛋白的相对分子质量及其分布的方法。使用凝胶色谱仪，TSKgel G3000SWXL型（300 mm×7.8 mm）高效凝胶排阻色谱柱，以0.05 mol/L磷酸盐缓冲溶液（pH 6.8）-0.3 mol/L氯化钠溶液为流动相，流速为1 mL/min，DAD检测器，检测波长为220 nm，选择300～330 nm作为参比波长，信号采集时间为15 min。测得质控样牛血清白蛋白相对分子质量标准物质峰值相对分子质量的均值为7.160×104 Da，与参考值的相对误差为7.76%，组间相对标准偏差为0.49%。采用该法对市售牛血清白蛋白样品的相对分子质量分布进行测试，组内相对标准偏差为0.004%～0.014%，组间相对标准偏差为1.89%。该法可以满足一般实验室对牛血清白蛋白样品的分子量分布测定的要求。
건립료일충고효응효배조색보쾌속측정우혈청백단백적상대분자질량급기분포적방법。사용응효색보의，TSKgel G3000SWXL형（300 mm×7.8 mm）고효응효배조색보주，이0.05 mol/L린산염완충용액（pH 6.8）-0.3 mol/L록화납용액위류동상，류속위1 mL/min，DAD검측기，검측파장위220 nm，선택300～330 nm작위삼비파장，신호채집시간위15 min。측득질공양우혈청백단백상대분자질량표준물질봉치상대분자질량적균치위7.160×104 Da，여삼고치적상대오차위7.76%，조간상대표준편차위0.49%。채용해법대시수우혈청백단백양품적상대분자질량분포진행측시，조내상대표준편차위0.004%～0.014%，조간상대표준편차위1.89%。해법가이만족일반실험실대우혈청백단백양품적분자량분포측정적요구。
The determination method of relative molecular mass and its distribution of bovine serum albumin (BSA) by high performance size exclusion chromatography was established. Chromatographic experiments conditions were as the following:TSK gel G3000SWXL column (300 mm×7.8 mm), a mobile phase of phosphate buffer solution (0.05 mol/L, pH 6.8) with 0.3 mol/L sodium chloride, DAD detector with the detection wavelength of 220 nm, 300-330 nm as the reference wavelength, the flow rate was 1.0 mL/min,the testing interval was 15 min. MP of the standard material for quality control was 7.160×104 Da, the relative deviation between measured value and certified value of the standard quality control sample was 7.76%in average with inter-assay RSD of 0.49%. The relative molecular mass and distribution of BSA samples were tested, the intra-assay RSD ware 0.004%-0.014%, and the inter-assay RSD was 1.89%, which could reach the requirement for the lab to test the relative molecular mass and distribution of the BSA samples.