CAJ | 학술논문

根结线虫(Meloidogyne spp.)是一种世界性的植物病害。在前期研究中，通过利用生物信息学方法在线虫全基因组中预测了一些功能基因。本研究以预测到的线粒体ATP合成酶g亚基基因(Asg)序列设计特异引物克隆了南方根结线虫(M. incognita)的Asg基因(MiAsg)，对其序列进行了特征分析后，利用病毒诱导的基因沉默(VIGS)技术，将其导入番茄植株，然后接种M. incognita，研究MiAsg基因与根结线虫病害的关系。结果表明，克隆到的MiAsg基因与预测到的MiAsg基因相似性高达100%。接种M. incognita 60 d后，MiAsg基因沉默的番茄植株根结数分别比空载体对照减少了59.6%，比清水对照降低了59.5%。结果表明，MiAsg基因沉默对根结线虫病害具有很好的防控效果，也说明MiAsg基因可能参与线虫的致病性。
근결선충(Meloidogyne spp.)시일충세계성적식물병해。재전기연구중，통과이용생물신식학방법재선충전기인조중예측료일사공능기인。본연구이예측도적선립체ATP합성매g아기기인(Asg)서렬설계특이인물극륭료남방근결선충(M. incognita)적Asg기인(MiAsg)，대기서렬진행료특정분석후，이용병독유도적기인침묵(VIGS)기술，장기도입번가식주，연후접충M. incognita，연구MiAsg기인여근결선충병해적관계。결과표명，극륭도적MiAsg기인여예측도적MiAsg기인상사성고체100%。접충M. incognita 60 d후，MiAsg기인침묵적번가식주근결수분별비공재체대조감소료59.6%，비청수대조강저료59.5%。결과표명，MiAsg기인침묵대근결선충병해구유흔호적방공효과，야설명MiAsg기인가능삼여선충적치병성。
Root-knot nematode (Meloidogyne spp.) is a worldwide disease. In preliminary studies, we predict the number of functional genes of nematode in its genome using bioinformatics methods. In this study, we designed special primers based on the predicted mitochondrial ATP synthase g subunit gene (Asg) sequence and cloned the Asg gene in M. incognita (MiAsg) which was transferred into tomato plantlets using virus-induced gene silence (VIGS) technique after analyzing the sequence characters to investigate the relationship between MiAsg gene and the disease caused by root-knot nematode. The results showed that the similarity between the cloned MiAsg gene and the predicted Asg was as high as 100%. Sixty days after inoculation with M. incognita, the amounts of root knot on MiAsg gene silence-treated tomato plantlets decreased by 59.6% compared to that of the empty vector control, by 59.5%compared to that of the water control. The study revealed the MiAsg gene silence had a good effect on prevention and control of root-knot nematode disease, also showed the MiAsg gene may be involved in the pathogenesis of nematode.