CAJ | 학술논문

种子纯度在种子生产中的重要性日益提升，分子标记技术因其具有高效、稳定、可靠等优点越来越多地被应用到种子纯度鉴定中。本研究利用SSR分子标记技术对黄瓜杂交品系‘浙秀1号’及其两亲本之间的多态性进行了引物筛选和种子纯度鉴定研究。结果表明，在699对供选SSR引物中，有3对引物分别在‘浙秀1号’杂交种和其双亲之间表现为稳定的共显性，杂交种带型为双亲的互补带型，适合于杂交种纯度鉴定。经田间试验验证，引物稳定性良好，且与田间鉴定结果非常接近，可用于‘浙秀1号’杂交种种子纯度的鉴定，显示出SSR标记技术在黄瓜杂交种子纯度的室内快速检测中有广泛应用前景。
충자순도재충자생산중적중요성일익제승，분자표기기술인기구유고효、은정、가고등우점월래월다지피응용도충자순도감정중。본연구이용SSR분자표기기술대황과잡교품계‘절수1호’급기량친본지간적다태성진행료인물사선화충자순도감정연구。결과표명，재699대공선SSR인물중，유3대인물분별재‘절수1호’잡교충화기쌍친지간표현위은정적공현성，잡교충대형위쌍친적호보대형，괄합우잡교충순도감정。경전간시험험증，인물은정성량호，차여전간감정결과비상접근，가용우‘절수1호’잡교충충자순도적감정，현시출SSR표기기술재황과잡교충자순도적실내쾌속검측중유엄범응용전경。
The status of seed purity are increased in seed production. The molecular marker techniques are app-lied to the identification of seed purity increasingly with advantages of quick, stable and reliable etc. In this paper, the polymorphism of‘Zhexiu 1’ hybrids and its parental lines were analyzed with SSR marker system. The results showed that there are 3 pairs of SSR primers presenting stable codominance among 699 pairs of screened SSR primers, which were stable and polymorphic amplification profiles in hybrid and their parents. The bands of hybrid were complement types of their parents, which were adapted to examine purity of‘Zhexiu 1’ hybrid seeds, and were approximate to their field purity respectively. It suggested that SSR technique has wide prospective in hybrid seed purity test of‘Zhexiu 1’.