CAJ | 학술논문

利用重组自交系进行陆地棉（Gossypium hirsutum L.）棉籽油分含量和蛋白质含量的QTL定位
이용중조자교계진행륙지면（Gossypium hirsutum L.）면자유분함량화단백질함량적QTL정위
Identification of QTL for Cottonseed Oil and Protein Content in Upland Cotton (Gossypium hirsutum L.) Based on a RIL Population

万方数据

分子植物育种分子植物育種 분자식물육충
MOLECULAR PLANT BREEDING
2013年 5期 520-528 ,共9页

刘小芳%李俊文%余学科%石玉真%贾菲%孙福鼎%刘爱英%龚举武%商海红%巩万奎%王涛%邓化冰%袁有禄

류소방%리준문%여학과%석옥진%가비%손복정%류애영%공거무%상해홍%공만규%왕도%산화빙%원유록

陆地棉%重组自交系%棉籽油分%蛋白质%QTL 陸地棉%重組自交繫%棉籽油分%蛋白質%QTL
륙지면%중조자교계%면자유분%단백질%QTL
Gossypium hirsutum L.%Recombinant inbred lines%Cottonsee

本研究利用以0-153为父本和SGK9708为母本构建的196个陆地棉重组自交系(F6：8)为材料对棉籽油分含量和蛋白质含量进行了遗传分析和QTL定位。通过四个环境下的群体材料的棉籽油分含量和蛋白质含量分析表明棉籽油分含量和蛋白质含量均为典型数量性状，其中棉籽油分含量存在超低亲本的超亲分离，而其蛋白质含量呈现超高亲本的超亲分离。相关性分析显示棉籽油分含量和蛋白质含量呈极显著负相关，同步提高两者在棉籽的的含量较为困难。基于包含186个标记，总长827.84 cM，标记间平均距离4.45 cM，覆盖棉花基因组18.6%的遗传连锁图谱，应用WinQTLcart2.5软件对四个环境下的棉籽油分含量和蛋白质含量进行了QTL定位，共检测到8个油分含量QTLs，解释表型变异5.42%~13.15%，其中稳定的QTL1个。4个蛋白质含量QTLs，解释表型变异4.35%~14.93%。本研究结果可为进行陆地棉种子营养品质性状的分子遗传改良奠定基础。
본연구이용이0-153위부본화SGK9708위모본구건적196개륙지면중조자교계(F6：8)위재료대면자유분함량화단백질함량진행료유전분석화QTL정위。통과사개배경하적군체재료적면자유분함량화단백질함량분석표명면자유분함량화단백질함량균위전형수량성상，기중면자유분함량존재초저친본적초친분리，이기단백질함량정현초고친본적초친분리。상관성분석현시면자유분함량화단백질함량정겁현저부상관，동보제고량자재면자적적함량교위곤난。기우포함186개표기，총장827.84 cM，표기간평균거리4.45 cM，복개면화기인조18.6%적유전련쇄도보，응용WinQTLcart2.5연건대사개배경하적면자유분함량화단백질함량진행료QTL정위，공검측도8개유분함량QTLs，해석표형변이5.42%~13.15%，기중은정적QTL1개。4개단백질함량QTLs，해석표형변이4.35%~14.93%。본연구결과가위진행륙지면충자영양품질성상적분자유전개량전정기출。
In the study, the population of 196 recombinant inbred lines (F6:8) of upland cotton derived from the cross of 0-153 and SGK9708 was used to analyze heredity and identify quantitative trait loci (QTLs) for cotton-seed oil and protein contents. The analysis of oil and protein in cottonseed in 4 environments indicates that oil and protein contents in cottonseed are typical quantitative traits, among which oil in cottonseed existed transgressive segregations lower than the low parent, while cottonseed protein content showed transgressive segregations higher than the high parent. The correlation analysis in the four environments displayed that oil content was negatively correlated with protein content in great significance, which indicates that it is difficult to improve the content of oil and protein in cottonseed simultaneously. Based on a genetic map of 186 SSR markers and a total genetic distance of 827.84 cM with an average interval of 4.45 cM between two markers and covering about 18.6% of cotton genome, 8 QTLs were detected for oil contents and contributed to the phenotypic variation (PV) by 5.42%~13.15%,in which 1 QTL was identified stably. 4 QTLs were detected for protein contents and contributed to the phenotypic variation (PV) by 4.35%~14.93%. The results lay the foundation for molecular genetic improvement of nutritional quality of upland cottonseed.