CAJ | 학술논문

利用生物信息学手段，结合公共大豆基因组数据库和大豆发育表达芯片数据获得大豆GST家族基因序列、蛋白序列和染色体位置等信息并进一步对基因的组织表达等进行分析。结果显示大豆中含94个GST家族基因，根据系统发育分析将这些GST基因分成5个亚家族；定位分析表明，94个GST基因分布于大豆的16条染色体上。表达分析结果表明，14个不同发育阶段，大多成员至少在一个组织中表达，11差异表达的基因中有7在根中表达，另外4在其它部位优势表达，基因表达具有一定特异性。本研究为进一步研究GST家族的功能及其抗逆利用提供基础。
이용생물신식학수단，결합공공대두기인조수거고화대두발육표체심편수거획득대두GST가족기인서렬、단백서렬화염색체위치등신식병진일보대기인적조직표체등진행분석。결과현시대두중함94개GST가족기인，근거계통발육분석장저사GST기인분성5개아가족；정위분석표명，94개GST기인분포우대두적16조염색체상。표체분석결과표명，14개불동발육계단，대다성원지소재일개조직중표체，11차이표체적기인중유7재근중표체，령외4재기타부위우세표체，기인표체구유일정특이성。본연구위진일보연구GST가족적공능급기항역이용제공기출。
Based on bioinformatic methods, soybean genome database and expression microarray data, soybean GST genes family were identified in this study. And their gene sequences, protein sequences and chromosome location were obtained. All of ninty-four GST genes from soybean were classified into 5 subfamilies according to phylogenetic relationship. And they were distributed on 16 chromosomes. Based on the soybean developmental expression database, the expression pattern of the GST gene family at different development organs and stages was analyzed. Eleven differentially expressed genes were found, and seven of them were highly expressed in root at each developmental stage by microarray. They were differentially expressed in different tissues and developmental stages. These results are helpful for their functional analysis and using for crop anti-stress improvement.