茶叶科学
茶葉科學
다협과학
2013年
5期
397-404
,共8页
王雪敏%马建强%金基强%马春雷%姚明哲%陈亮
王雪敏%馬建彊%金基彊%馬春雷%姚明哲%陳亮
왕설민%마건강%금기강%마춘뢰%요명철%진량
茶树%杂交一代%生化成分%儿茶素类%生物碱
茶樹%雜交一代%生化成分%兒茶素類%生物堿
다수%잡교일대%생화성분%인다소류%생물감
tea plant%first generation%biochemical composition%catechins%alkaloids
对茶×白毛茶人工杂交一代229个单株儿茶素类和生物碱采用高效液相色谱法进行检测。结果表明,茶树杂交一代生化成分存在丰富的多样性和变异,平均遗传多样性指数达到2.02,平均变异系数达到27.51%。F1个体的儿茶素品质指数为348.39~2583.96,平均值为762.47。通过多变量的主成分分析,前5个主成分代表了茶树生化成分多样性86.94%的信息。基于生化成分,将亲本及229个单株聚为2个类群,第1类群100个单株,第2类群131个单株,包括2个亲本。对茶树人工杂交后代儿茶素类和生物碱遗传规律的深入研究,可为茶树育种鉴定与利用提供参考。
對茶×白毛茶人工雜交一代229箇單株兒茶素類和生物堿採用高效液相色譜法進行檢測。結果錶明,茶樹雜交一代生化成分存在豐富的多樣性和變異,平均遺傳多樣性指數達到2.02,平均變異繫數達到27.51%。F1箇體的兒茶素品質指數為348.39~2583.96,平均值為762.47。通過多變量的主成分分析,前5箇主成分代錶瞭茶樹生化成分多樣性86.94%的信息。基于生化成分,將親本及229箇單株聚為2箇類群,第1類群100箇單株,第2類群131箇單株,包括2箇親本。對茶樹人工雜交後代兒茶素類和生物堿遺傳規律的深入研究,可為茶樹育種鑒定與利用提供參攷。
대다×백모다인공잡교일대229개단주인다소류화생물감채용고효액상색보법진행검측。결과표명,다수잡교일대생화성분존재봉부적다양성화변이,평균유전다양성지수체도2.02,평균변이계수체도27.51%。F1개체적인다소품질지수위348.39~2583.96,평균치위762.47。통과다변량적주성분분석,전5개주성분대표료다수생화성분다양성86.94%적신식。기우생화성분,장친본급229개단주취위2개류군,제1류군100개단주,제2류군131개단주,포괄2개친본。대다수인공잡교후대인다소류화생물감유전규률적심입연구,가위다수육충감정여이용제공삼고。
The contents of catechins and alkaloids of 229 individuals in the combination of Camellia sinensis (L.) O. Kuntze ×C. sinensis var. pubilimba Chang were detected by using high performance liquid chromatographic (HPLC). The results showed that the biochemical diversity and variation were high in first generation of tea plants. The average diversity index and coefficient of variation were 2.02 and 27.51%, respectively. The catechin quality index ranged from 348.39~2 583.96, with average of 762.47. In principal component analysis, the first five principal components represented 86.94%of the biochemical diversity. Based on the biochemical data, 229 individuals of first generation and parents were clustered into two groups, 100 individuals in group one, 131 individuals in group two, and both parents were clustered in group two. It provided the reference for tea breeding identification and utilization in the future.