CAJ | 학술논문

利用自设寡核苷酸探针，通过分析黄曲霉产毒基因簇中aflR基因，建立黄曲霉毒素的SPR快速检测技术，其阴性对照链检测到SPR角度改变值，确立检测结果的准确性。同时建立SPR检测aflR基因的标准曲线，并检测到不同时期黄曲霉D1中aflR基因的表达量，发现该基因随菌株培养时间的延长，其表达量下降。
이용자설과핵감산탐침，통과분석황곡매산독기인족중aflR기인，건립황곡매독소적SPR쾌속검측기술，기음성대조련검측도SPR각도개변치，학립검측결과적준학성。동시건립SPR검측aflR기인적표준곡선，병검측도불동시기황곡매D1중aflR기인적표체량，발현해기인수균주배양시간적연장，기표체량하강。
In this paper, the detection method for aflatoxin with SPR (Surface plasmon resonance) is constructed, through analysis of aflR gene coming from aflatoxin producing gene cluster, and its negative control shows the change of SPR angle, which means the correction of detection results. The standard curve of aflR gene detection is also constructed. And we also find the changes of aflR gene in different culturing stage that is the quantity of aflR gene decreased with culturing time.