CAJ | 학술논문

目的：建立超微戊己丸中吴茱萸碱和吴茱萸次碱的含量测定方法。方法采用Kromasail C18色谱柱（250mm×4.6mm，5μm），流动相为流速1.0mL/min的乙腈-乙腈10%（50：50），测定波长为225nm。结果吴茱萸碱和吴茱萸次碱理论板数分别为2681和2067，Y吴茱萸碱=1.965×10-7X +0.07646,r=0.9999）, Y吴茱萸次碱=3.658×10-7X-0.2199,r=0.9999）,吴茱萸碱与吴茱萸次碱的线性区间分别为10.2～51.0μg/mL以及10.0～50.0μg/mL ，两者平均回收率分别为97.3%和101.4%，其相对标准偏差分别为3.2%和3.9%，最低检测限为0.05μg/mL和0.1μg/mL。结论使用高效液相色谱法对超微戊己丸当中的吴茱萸碱以及吴茱萸次碱含量进行检测的精确率较高，重现性好。
목적：건립초미무기환중오수유감화오수유차감적함량측정방법。방법채용Kromasail C18색보주（250mm×4.6mm，5μm），류동상위류속1.0mL/min적을정-을정10%（50：50），측정파장위225nm。결과오수유감화오수유차감이론판수분별위2681화2067，Y오수유감=1.965×10-7X +0.07646,r=0.9999）, Y오수유차감=3.658×10-7X-0.2199,r=0.9999）,오수유감여오수유차감적선성구간분별위10.2～51.0μg/mL이급10.0～50.0μg/mL ，량자평균회수솔분별위97.3%화101.4%，기상대표준편차분별위3.2%화3.9%，최저검측한위0.05μg/mL화0.1μg/mL。결론사용고효액상색보법대초미무기환당중적오수유감이급오수유차감함량진행검측적정학솔교고，중현성호。
Objective To establish a method for measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills. Methods Chromatographic column: Kromasail C18(250mm×4.6mm, 5μm); mobile phase:acetonitrile-acetonitrile(50:50); flow rate: 1.0mL/min; wavelength: 225nm. Results The number of theoretical plates for evodiamine and rutaecarpin was 2681 and 2067 respectively(Yevodiamine=1.965×10-7X+0.07646, r=0.9999;Yrutaecarpin=3.658×10-7X-0.2199, r=0.9999). Evodiamine and rutaecarpin had linear interval of 10.2-51.0μg/mL and 10.0-50.0μg/mL respectively, average recovery rate of 97.3% and 101.4% respectively, relative standard deviation of 3.2% and 3.9% respectively, lowest detection limit of 0.05μg/mL and 0.1μg/mL respectively. Conclusion High performance liquid chromatography demonstrates high accuracy and good reproducibility for measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills.