CAJ | 학술논문

目的观察脓毒症大鼠的肺组织中c-FLIP的变化情况,并探讨乌司他丁对c-FLIP水平的影响.方法将30只雄性Sprague-Dawley大鼠随机(随机数字法)分为假手术组(Sham组)、脓毒症模型组(CLP组)及乌司他丁治疗组(UTI组),每组10只.Sham组不结扎穿刺盲肠,余同CLP组;CLP组采用肓肠结扎并穿刺法造模;UTI组造模后立即经阴茎静脉注射乌司他丁注射液(50000 U/kg).12 h后处死大鼠并采取肺组织,光镜下观察形态学变化;Western-Blot法检测c-FLIP蛋白表达,RT-PCR法检测c-FLIP基因表达.应用SPSS 11.5进行统计学分析,多组比较采用方差分析,两两比较采用LSD-t检验.结果脓毒症组光镜下肺泡腔内中性粒细胞和大量红细胞渗出,c-FLIP蛋白和基因表达较假手术组显著降低(P＜0.05);用乌司他丁治疗后,光镜下病理改变减轻,c-FLIP蛋白和基因表达较脓毒症组显著升高(P＜0.05).结论脓毒症中c-FLIP在肺组织中的表达下降,提示肺组织的细胞凋亡增加.乌司他丁能上调脓毒症大鼠肺组织中c-FLIP的表达.
목적 관찰농독증대서적폐조직중c-FLIP적변화정황,병탐토오사타정대c-FLIP수평적영향.방법 장30지웅성Sprague-Dawley대서수궤(수궤수자법)분위가수술조(Sham조)、농독증모형조(CLP조)급오사타정치료조(UTI조),매조10지.Sham조불결찰천자맹장,여동CLP조;CLP조채용황장결찰병천자법조모;UTI조조모후립즉경음경정맥주사오사타정주사액(50000 U/kg).12 h후처사대서병채취폐조직,광경하관찰형태학변화;Western-Blot법검측c-FLIP단백표체,RT-PCR법검측c-FLIP기인표체.응용SPSS 11.5진행통계학분석,다조비교채용방차분석,량량비교채용LSD-t검험.결과 농독증조광경하폐포강내중성립세포화대량홍세포삼출,c-FLIP단백화기인표체교가수술조현저강저(P＜0.05);용오사타정치료후,광경하병리개변감경,c-FLIP단백화기인표체교농독증조현저승고(P＜0.05).결론 농독증중c-FLIP재폐조직중적표체하강,제시폐조직적세포조망증가.오사타정능상조농독증대서폐조직중c-FLIP적표체.
Objective To explore the changes of e-FLIP in the lung of rats with sepsis,and to investigate the effect of Ulinastatin on the level of c-FLIP.Method Thirty male Spragne-Dawley rats were randomly(random number)divided into 3 groups:sham operated group(Sham),sepsis group(CLP)and Ulinastatin treated group (UTI),n=10,respectively.In Sham group,only laparotomy was performed.In CLP and UTI groups,cecal ligation and puncture was performed to induce sepsis.Ulinastatin(50 000 U/kg)was administered intravenously via penile vein in UTI group after operation.The same volume of normal saune instead of Ulinastatin was administered intravenously in Sham and CLP groups.The lungs of rats were harvested 12 hours after operation for determination of c-FLIP mRNA expressions assayed by using RT-PCR and cFLIP protein measured by Western blot.Statistical analysis was performed with SPSS version 11.5 software.ANOVA was used for multiple groups comparison,and t-test for paired comparison.Results Compared with Sham group,there was a significant decrease in c-FLIP protein level and c-FLIP mRNA expression in lung(P＜0.05).After treatment with Ulinastatin,the level of c-FLIP protein and mRNA expression obviously increased compared with CLP group(P＜0.05).Conclusions The levels of c-FLIP decreased in lung of rats with sepsis,which suggests that pulmonary apoptosis might increase.Ulinastatin can increase the c-FLIP levels in the lung.