CAJ | 학술논문

目的：研究VHL基因对U251胶质瘤细胞侵袭和迁移能力的影响。方法：使用VHL表达质粒转染胶质瘤细胞，采用RT-PCR检测VHL mRNA表达，Western blot检测VHL、MMP-2、MMP-9蛋白表达，应用Transwell体外侵袭实验、划痕实验检测上调VHL基因后对U251胶质瘤细胞侵袭迁移能力的影响。使用VHL表达质粒处理后的U251细胞建立裸鼠颅内模型，利用免疫组织化学染色法对颅内模型组织切片中VHL、MMP-2、MMP-9蛋白表达进行检测。结果：VHL表达质粒转染胶质瘤细胞后VHL mRNA、VHL蛋白表达增高，MMP-2、MMP-9蛋白表达下降；并抑制U251胶质瘤细胞侵袭迁移能力。对染色后的组织切片进行分析，VHL表达质粒处理组中VHL的表达较对照组明显升高，MMP-2、MMP-9蛋白较对照组表达减少。结论：VHL基因能够有效抑制U251胶质瘤细胞侵袭迁移能力，VHL基因可成为治疗胶质瘤的一个有效靶点。
목적：연구VHL기인대U251효질류세포침습화천이능력적영향。방법：사용VHL표체질립전염효질류세포，채용RT-PCR검측VHL mRNA표체，Western blot검측VHL、MMP-2、MMP-9단백표체，응용Transwell체외침습실험、화흔실험검측상조VHL기인후대U251효질류세포침습천이능력적영향。사용VHL표체질립처리후적U251세포건립라서로내모형，이용면역조직화학염색법대로내모형조직절편중VHL、MMP-2、MMP-9단백표체진행검측。결과：VHL표체질립전염효질류세포후VHL mRNA、VHL단백표체증고，MMP-2、MMP-9단백표체하강；병억제U251효질류세포침습천이능력。대염색후적조직절편진행분석，VHL표체질립처리조중VHL적표체교대조조명현승고，MMP-2、MMP-9단백교대조조표체감소。결론：VHL기인능구유효억제U251효질류세포침습천이능력，VHL기인가성위치료효질류적일개유효파점。
To determine the effects of Von Hippel-Lindau (VHL) on the invasion and migration of glioma U251 cells. Methods:U251 GBM cells were transfected using VHL expression plasmid. Real-time polymerase chain reaction was conducted to de-tect VHL mRNA expression after transfection. Western blot assay was used to measure protein (VHL, MMP-2, and MMP-9) expres-sion. Tumor invasion and migration were examined by the Transwell and wound-healing experimental methods after VHL up-regula-tion. The intracranial model of nude mouse was developed using U251 cells transfected by VHL expression plasmid, and immunohisto-chemical staining was used to measure protein (VHL, MMP-2, and MMP-9) expression in the tissue sections. Results: In the U251 cells transfected by VHL expression plasmid, the expression of VHL mRNA and VHL proteins increased, and the expression of MMP-2 and MMP-9 protein decreased. Meanwhile, the invasion and migration of glioma U251 cells were also inhibited. Immunohistochemical staining results showed that the expression of MMP-2 and MMP-9 proteins decreased, and the VHL protein expression increased after transfection. Conclusion:VHL can inhibit the invasion and migration of glioma U251 cells. Thus, VHL gene can be used as a target for the gene therapy of gliomas.