CAJ | 학술논문

为确定猪尿中盐酸赛庚啶的残留指示物，将15头生猪分成3组，饲养环境相同，A组为空白对照组，B组为低剂量盐酸赛庚啶组（10 mg/d），C组为高剂量盐酸赛庚啶组（100 mg/d）。对3组同时进行7 d的加量饲喂以及7d的停止饲喂体内消除实验。优化了葡萄糖醛酸酶和硫酸芳基酯酶对猪尿进行酶解反应的条件，用已经建立的UPLC-MS/MS法分别测定了酶解与非酶解的猪尿样品中盐酸赛庚啶原型药物的含量，研究猪尿中原型药物与其衍生物的积累和消除规律。实验结果表明，猪尿中盐酸赛庚啶主要以原型药物的形式存在。其含量与饲喂量呈正相关，半衰期为2 d，6 d后几乎完全消除。
위학정저뇨중염산새경정적잔류지시물，장15두생저분성3조，사양배경상동，A조위공백대조조，B조위저제량염산새경정조（10 mg/d），C조위고제량염산새경정조（100 mg/d）。대3조동시진행7 d적가량사위이급7d적정지사위체내소제실험。우화료포도당철산매화류산방기지매대저뇨진행매해반응적조건，용이경건립적UPLC-MS/MS법분별측정료매해여비매해적저뇨양품중염산새경정원형약물적함량，연구저뇨중원형약물여기연생물적적루화소제규률。실험결과표명，저뇨중염산새경정주요이원형약물적형식존재。기함량여사위량정정상관，반쇠기위2 d，6 d후궤호완전소제。
To determine the residual indicator of cyproheptadine in swine urine,15 healthy swine were selected and divided into 3 groups with the same breeding environment.Group A was fed with blank feed,while group B was fed with low content(10 mg/d)and group C was fed with high content(100 mg/d).The 7 days accumulation test and 7 days elimina-tion test was held.With the glucuronidase and the sulfuricarylesterase reaction optimized as well as the UPLC-MS/MS method established,the concentration of cyproheptadine hydrochloride both enzymolyzed and unenzymolyzed was analyzed. The accumulation rule and the elimination rule were both determined.According to the result,cyproheptadine hydrochloride existed in swine urine mainly in the form of original drug.Significant correlation existed between the content and the feeding amount.The drug has a half-life of 2 days,and it almost completely eliminated after 6 days.