CAJ | 학술논문

目的：检测肝脏X受体(Liver X receptor alpha，LXRα)在HBV阳性肝硬化组织及原发性肝癌中的表达情况及临床意义。方法收集20例揭阳市人民医院及汕头大学医学院第一附属医院手术切除的HBV阳性肝硬化原发性肝癌组织标本，应用免疫组化染色检测LXRα表达。Huh7细胞中分别过表达小鼠mLXRα，及共过表达HBx (HBV X protein)和mLXRα，并检测mLXRα及HBx对内源性LXRα信号通路下游靶基因SREBP-1(Sterol regulatory element-binding protein 1)的影响；双荧光素酶报告系统检测人肝癌Huh7细胞中HBx对LXRα转录活性的影响。结果 LXRα在肝硬化组织中阳性表达率为95%，高于癌组织的25%。HBx上调SREBP-1的表达可能与HBx调控LXRα的转录活性有关。结论 HBx通过调节癌前高表达的LXRα的转录活性，并影响其信号通路可能与HCC发生相关。
목적：검측간장X수체(Liver X receptor alpha，LXRα)재HBV양성간경화조직급원발성간암중적표체정황급림상의의。방법수집20례게양시인민의원급산두대학의학원제일부속의원수술절제적HBV양성간경화원발성간암조직표본，응용면역조화염색검측LXRα표체。Huh7세포중분별과표체소서mLXRα，급공과표체HBx (HBV X protein)화mLXRα，병검측mLXRα급HBx대내원성LXRα신호통로하유파기인SREBP-1(Sterol regulatory element-binding protein 1)적영향；쌍형광소매보고계통검측인간암Huh7세포중HBx대LXRα전록활성적영향。결과 LXRα재간경화조직중양성표체솔위95%，고우암조직적25%。HBx상조SREBP-1적표체가능여HBx조공LXRα적전록활성유관。결론 HBx통과조절암전고표체적LXRα적전록활성，병영향기신호통로가능여HCC발생상관。
Objective To detect the expression of liver X receptor alpha (LXRα) in HBV infection associat-ed liver cirrhosis and hepatocellular carcinoma tissues and explore its clinical significance. Methods The expression of LXRαin 20 cases of HBV infection-associated liver cirrhosis and hepatocellular carcinoma specimens from Jiey-ang People's Hospital and the First Affiliated Hospital of Medical College, Shantou University was detected by immu-nohistochemical staining. Endogenous SREBP-1, a typical LXRα target gene, was detected by real-time PCR after over-expressing mice mLXRα, HBx and mLXRαin Huh7 cells. The transcriptional regulation of LXRαsignaling by HBx was evaluated by dual luciferase reporter system. Results The positive expression rate of LXRαwas 95%in cirrhosis, which was higher than that in HCC tissue with only 25%. The up-regulation of SREBP-1 expression by HBx may be related with the transcriptional activation of LXRαby HBx. Conclusion HBx maybe associated with the development of HCC by regulation of the transcriptional activation of LXRa, which was higher in cirrhosis, and ac-tivating the signal pathway.