中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
21期
23-25,26
,共4页
直肠癌%Ghrelin%MMP-9%淋巴转移
直腸癌%Ghrelin%MMP-9%淋巴轉移
직장암%Ghrelin%MMP-9%림파전이
Rectal cancer%Ghrelin%MMP-9%Lymphatic metastasis
目的：探讨直肠癌组织中生长激素释放肽（Ghrelin）和基质金属蛋白酶-9（MMP-9）的表达与淋巴结转移的关系。方法：应用免疫组织化学方法及Western blot检测Ghrelin和MMP-9在21例无淋巴结转移及35例有淋巴结转移直肠癌组织中的表达水平的表达，分析Ghrelin和MMP-9与直肠癌淋巴结转移中的关系及两者的相关性。结果：Ghrelin和MMP-9在直肠癌组织中的阳性表达率分别为53.57%和80.36%，明显高于癌旁正常组织（P<0.05）；Ghrelin和MMP-9蛋白表达与肿瘤淋巴结转移比率联系密切，并且在直肠癌组织中的阳性表达随着淋巴结转移个数增加而增加（P<0.05）；Ghrelin与MMP-9的阳性表达呈正相关（κ=0.832，P<0.001）。结论：Ghrelin和MMP-9表达升高与直肠癌的淋巴结转移呈正相关，联合检测Ghrelin和MMP-9可能对直肠癌患者淋巴结转移及预后评估具有重要临床价值。
目的：探討直腸癌組織中生長激素釋放肽（Ghrelin）和基質金屬蛋白酶-9（MMP-9）的錶達與淋巴結轉移的關繫。方法：應用免疫組織化學方法及Western blot檢測Ghrelin和MMP-9在21例無淋巴結轉移及35例有淋巴結轉移直腸癌組織中的錶達水平的錶達，分析Ghrelin和MMP-9與直腸癌淋巴結轉移中的關繫及兩者的相關性。結果：Ghrelin和MMP-9在直腸癌組織中的暘性錶達率分彆為53.57%和80.36%，明顯高于癌徬正常組織（P<0.05）；Ghrelin和MMP-9蛋白錶達與腫瘤淋巴結轉移比率聯繫密切，併且在直腸癌組織中的暘性錶達隨著淋巴結轉移箇數增加而增加（P<0.05）；Ghrelin與MMP-9的暘性錶達呈正相關（κ=0.832，P<0.001）。結論：Ghrelin和MMP-9錶達升高與直腸癌的淋巴結轉移呈正相關，聯閤檢測Ghrelin和MMP-9可能對直腸癌患者淋巴結轉移及預後評估具有重要臨床價值。
목적：탐토직장암조직중생장격소석방태（Ghrelin）화기질금속단백매-9（MMP-9）적표체여림파결전이적관계。방법：응용면역조직화학방법급Western blot검측Ghrelin화MMP-9재21례무림파결전이급35례유림파결전이직장암조직중적표체수평적표체，분석Ghrelin화MMP-9여직장암림파결전이중적관계급량자적상관성。결과：Ghrelin화MMP-9재직장암조직중적양성표체솔분별위53.57%화80.36%，명현고우암방정상조직（P<0.05）；Ghrelin화MMP-9단백표체여종류림파결전이비솔련계밀절，병차재직장암조직중적양성표체수착림파결전이개수증가이증가（P<0.05）；Ghrelin여MMP-9적양성표체정정상관（κ=0.832，P<0.001）。결론：Ghrelin화MMP-9표체승고여직장암적림파결전이정정상관，연합검측Ghrelin화MMP-9가능대직장암환자림파결전이급예후평고구유중요림상개치。
To investigate the correlation between the Ghrelin and MMP-9 in rectal cancer tissue with lymph node metastasis.Method:A total of 21 cases of rectal cancer without lymphatic metastasis and 35 cases of rectal cancer with lymphatic metastasis were enrolled in this study.Immunohistochemical staining and western blot were performed to detect the expression of Ghrelin and MMP-9 in the tumor tissues of 56 patients.And statistical analysis technique was used to investigate the relation between their expression and the relationship between the expression of Ghrelin and MMP-9.Result:The expression of Ghrelin and MMP-9 in rectal cancer tissue was 53.57%and 80.36%,respectively, they were significantly highter than that in the adjacent mucosal tissues(P<0.05).There were a close correlation between the expression of Ghrelin and MMP-9 with lymph node metastasis rate(P<0.05).Furthermore,the positive expression of Ghrelin and MMP-9 increased with increasing lymphatic metastasis.The positive expression of Ghrelin was consistent with the positive expression of MMP-9(κ=0.832,P<0.001).Conclusion:Expressions of Ghrelin and MMP-9 are closely related to the degree of lymphatic metastasis of rectal cancer,and their combination may be an important indicator to evaluate the malignant degree and prognosis of the rectal cancer.
