医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2014年
22期
100-100
,共1页
E3泛素连接酶%卵巢癌%真核表达质粒
E3汎素連接酶%卵巢癌%真覈錶達質粒
E3범소련접매%란소암%진핵표체질립
Homologous to the E6-associated protein carboxyl terminus domain containing 3 (HETCD3)%Ovarian cancer%Eukaryotic expression plasmid
目的：鉴定E3泛素连接酶(homologous to the E6-associated protein carboxyl terminus domain containing 3，HECTD3)基因的真核表达质粒。方法提取无内毒素真核表达质粒pcDNA-HECTD3，利用lipofectamine 2000将其转染进卵巢癌细胞SKOV-3细胞，qRT-PCR法和蛋白质免疫印迹技术检测该基因的表达情况。结果真核表达质粒pcDNA-HECTD3可以显著地提高HECTD3基因在mRNA和蛋白水平的表达量。结论 HECTD3基因的真核表达质粒构建成功，可以用于后续卵巢癌发生发展的分子生物学研究。
目的：鑒定E3汎素連接酶(homologous to the E6-associated protein carboxyl terminus domain containing 3，HECTD3)基因的真覈錶達質粒。方法提取無內毒素真覈錶達質粒pcDNA-HECTD3，利用lipofectamine 2000將其轉染進卵巢癌細胞SKOV-3細胞，qRT-PCR法和蛋白質免疫印跡技術檢測該基因的錶達情況。結果真覈錶達質粒pcDNA-HECTD3可以顯著地提高HECTD3基因在mRNA和蛋白水平的錶達量。結論 HECTD3基因的真覈錶達質粒構建成功，可以用于後續卵巢癌髮生髮展的分子生物學研究。
목적：감정E3범소련접매(homologous to the E6-associated protein carboxyl terminus domain containing 3，HECTD3)기인적진핵표체질립。방법제취무내독소진핵표체질립pcDNA-HECTD3，이용lipofectamine 2000장기전염진란소암세포SKOV-3세포，qRT-PCR법화단백질면역인적기술검측해기인적표체정황。결과진핵표체질립pcDNA-HECTD3가이현저지제고HECTD3기인재mRNA화단백수평적표체량。결론 HECTD3기인적진핵표체질립구건성공，가이용우후속란소암발생발전적분자생물학연구。
Objective To confirm the eukaryotic expression plasmid containing the gene of homologous to the E6-associated protein carboxyl terminus domain containing 3 (HECTD3). Methods Extracted the endo-free plasmid pcDNA-HECTD3 and transfected it into ovarian cellSKOV-3, final y qRT-PCR and western blot assay were used to measure HECTD3 expression levels. Results HECTD3 gene was remarkably overexpressed in transfected cells. Conclusion The HECTD3 gene expression plasmid can be the tools for further research of ovarian cancer.
