CAJ | 학술논문

目的：观察芪参益气滴丸对鸡胚绒毛尿囊膜血管生成的影响，测定其对人脐静脉内皮细胞增殖的影响，探讨该药促进血管生成的可能机制。方法实验分组：生理盐水组、空白血清组、bFGF 组、芪参益气滴丸组；建立鸡胚尿囊膜（CAM）模型，计数各组 CAM 上血管数；体外培养人脐静脉内皮细胞，应用血管内皮细胞增殖实验（MTT）法测定各组 A 值。结果与生理盐水组及空白血清组比较，芪参益气滴丸组具有明显的促 CAM 血管生成作用（P ＜0．05），并促进人脐静脉内皮细胞增殖（P ＜0．05）。结论芪参益气滴丸具有促血管生成作用，其机制可能与该药促进内皮细胞增殖有关。
목적：관찰기삼익기적환대계배융모뇨낭막혈관생성적영향，측정기대인제정맥내피세포증식적영향，탐토해약촉진혈관생성적가능궤제。방법실험분조：생리염수조、공백혈청조、bFGF 조、기삼익기적환조；건립계배뇨낭막（CAM）모형，계수각조 CAM 상혈관수；체외배양인제정맥내피세포，응용혈관내피세포증식실험（MTT）법측정각조 A 치。결과여생리염수조급공백혈청조비교，기삼익기적환조구유명현적촉 CAM 혈관생성작용（P ＜0．05），병촉진인제정맥내피세포증식（P ＜0．05）。결론기삼익기적환구유촉혈관생성작용，기궤제가능여해약촉진내피세포증식유관。
Objective To observe the influence of Qishenyiqi dripping pills in chick chorioallantoic membrane angio -genesis.Measured in human umbilical vein endothelial cell proliferation ,and investigate the possible mechanisms Qishenyiqi dripping pills promote angiogenesis .Methods Experimental groups:saline group,blank serum,bFGF group,dropping pills Qishenyiqi group.Establish chorioallantoic model .Counting the number of vessels in each group on the chorioallantoic model . Cultured human umbilical vein endothelial cells ,A value was determined by MTT.Results The Qishenyiqi dripping pills group could significantly promote the angiogenesis effect of CAM than that of normal saline group and blank serum group ,and could promote the proliferation of human umbilical vein endothelial cells ,the difference was statistically significant (P <0.05). Conclusion The Qishenyiqi dripping pills group can significantly promote the growth of vascular ,the possible mechanisms is promote the endothelial cell proliferation .