CAJ | 학술논문

目的：比较强直性脊柱炎患者HLA-B27常用的三种检测方法的特点，探讨不同检测方法之间差异及各自优缺点，为临床检验提供参考。方法：采用ELIsA法、RT-pCR法和流式细胞法，对120例强直性脊柱炎患者和100例正常对照人群进行HLA-B27检测，并对结果进行分析。结果：ELIsA法、RT-pCR法和流式细胞法三种检测方法的敏感性、特异性和阳性、阴性预测值分别为83.3%、94.0%、94.3%、82.5%；95.8%、93.0%、94.3%、94.8%；93.3%、95.0%、95.7%、92.2%。结论：三种血清HLA-B27检测方法诊断As的特异度均较高；pCR法和流式细胞法的敏感度均显著高于ELIsA法，其中pCR法略高于流式细胞术。
목적：비교강직성척주염환자HLA-B27상용적삼충검측방법적특점，탐토불동검측방법지간차이급각자우결점，위림상검험제공삼고。방법：채용ELIsA법、RT-pCR법화류식세포법，대120례강직성척주염환자화100례정상대조인군진행HLA-B27검측，병대결과진행분석。결과：ELIsA법、RT-pCR법화류식세포법삼충검측방법적민감성、특이성화양성、음성예측치분별위83.3%、94.0%、94.3%、82.5%；95.8%、93.0%、94.3%、94.8%；93.3%、95.0%、95.7%、92.2%。결론：삼충혈청HLA-B27검측방법진단As적특이도균교고；pCR법화류식세포법적민감도균현저고우ELIsA법，기중pCR법략고우류식세포술。
Objective To compare three different detection methods of HLA-B27 in patients with ankylosing spondylitis and to explore the differences including their advantages and disadvantages of the three detection methods,to provide reference for clinical diagno-sis. Method The HLA-B27 of 120 cases of patients with ankylosing spondylitis and 100 healthy control subjects were detected by ELIsA, RT-pCR and flow cytometry method,and the results were analyzed. Results The sensitivity,specificity,and positive and negative predic-tive values by ELIsA,RT-pCR and flow cytometry method were 83. 3%,94. 0%,94. 3%,82. 5%;95. 8%,93. 0%,94. 3%,94. 8%;93. 3%,95. 0%,95. 7%,92. 2%. Conclusion Three specificity of serum HLA-B27 by all three methods are high;The sensitivity of pCR method and flow cytometry were significantly higher than ELIsA.