现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
23期
2524-2526,2535
,共4页
IL-1β%幽门括约肌%大鼠%NO
IL-1β%幽門括約肌%大鼠%NO
IL-1β%유문괄약기%대서%NO
IL-1β%pyloric sphincter%rat%NO
目的：观察IL-1β对大鼠离体幽门括约肌自发收缩的影响，初步探讨其作用机制。方法成年SD大鼠（雌雄不拘）随机分为正常组、IL-1β组、亚甲基蓝组、L-NAME组。采用离体组织灌流的方法，用RM6240 C生物信号采集系统观察IL-1β对大鼠离体幽门括约肌自发收缩作用的影响；通过应用不同信号途径的特异性阻断剂，探讨IL-1β的作用机制。结果 IL-1β（终浓度为2，20，200μg/L）能抑制大鼠离体幽门括约肌自发收缩的振幅、频率和张力。亚甲基蓝（10μmol/L）和L-NAME（100μmol/L）能部分抵消IL-1β对大鼠离体幽门括约肌自发收缩的抑制作用，与IL-1β单独作用相比，振幅、频率和张力均较高（ P均＜0.01）。结论 IL-1β能抑制大鼠离体幽门括约肌的自发收缩，其作用可被亚甲基蓝、L-NAME部分阻断，说明其机制很可能与cGMP、NO有关。
目的：觀察IL-1β對大鼠離體幽門括約肌自髮收縮的影響，初步探討其作用機製。方法成年SD大鼠（雌雄不拘）隨機分為正常組、IL-1β組、亞甲基藍組、L-NAME組。採用離體組織灌流的方法，用RM6240 C生物信號採集繫統觀察IL-1β對大鼠離體幽門括約肌自髮收縮作用的影響；通過應用不同信號途徑的特異性阻斷劑，探討IL-1β的作用機製。結果 IL-1β（終濃度為2，20，200μg/L）能抑製大鼠離體幽門括約肌自髮收縮的振幅、頻率和張力。亞甲基藍（10μmol/L）和L-NAME（100μmol/L）能部分牴消IL-1β對大鼠離體幽門括約肌自髮收縮的抑製作用，與IL-1β單獨作用相比，振幅、頻率和張力均較高（ P均＜0.01）。結論 IL-1β能抑製大鼠離體幽門括約肌的自髮收縮，其作用可被亞甲基藍、L-NAME部分阻斷，說明其機製很可能與cGMP、NO有關。
목적：관찰IL-1β대대서리체유문괄약기자발수축적영향，초보탐토기작용궤제。방법성년SD대서（자웅불구）수궤분위정상조、IL-1β조、아갑기람조、L-NAME조。채용리체조직관류적방법，용RM6240 C생물신호채집계통관찰IL-1β대대서리체유문괄약기자발수축작용적영향；통과응용불동신호도경적특이성조단제，탐토IL-1β적작용궤제。결과 IL-1β（종농도위2，20，200μg/L）능억제대서리체유문괄약기자발수축적진폭、빈솔화장력。아갑기람（10μmol/L）화L-NAME（100μmol/L）능부분저소IL-1β대대서리체유문괄약기자발수축적억제작용，여IL-1β단독작용상비，진폭、빈솔화장력균교고（ P균＜0.01）。결론 IL-1β능억제대서리체유문괄약기적자발수축，기작용가피아갑기람、L-NAME부분조단，설명기궤제흔가능여cGMP、NO유관。
Objective It is to observe the effect of IL -1βon the spontaneous contractions of isolated rats ’ pyloric sphinc-ter in order to explore its mechanism .Methods Male or female SD rats were divided into 4 groups randomly:normal group , IL-1βgroup, methylene blue trihydrate group and L -NAME group, the effect of IL-1βon the spontaneous contractions of i-solated rats ’ pyloric sphincter was observed by RM 6240 C biologic signal selecting system , the mechanism of IL-1βwas ex-plored by specific repressor of different signal pathway .Results IL-1β(final concentration were 2, 20, 200μg/L) could in-hibit the amplitude , frequency and tension the spontaneous contractions of isolated rats ’ pyloric sphincter .Pretreatment of the tissues with methylene blue trihydrate (10μmol/L) and L-NAME (100μmol/L) markedly attenuated the inhibitory effects of IL-1β.The amplitude , frequency and tension were increased compared with methylene blue trihydrate absented .Pretreat-ment of the tissues with L -NAME (100μM) markedly attenuated the inhibitory effects of IL -1β.The amplitude, frequency and tension were increased compared with L -NAME absented (P<0.01).Conclusion IL-1βcan inhibit spontaneous con-tractions on rats ’ isolated pyloric sphincter .The inhibitory effects of IL -1βcan be attenuated by methylene blue trihydrate and L-NAME, which indicates that the mechanism may be related with the levels of NO and cGMP in intracellular .
