CAJ | 학술논문

目的：研究髓鞘碱性蛋白（ MBP）对实验性脑出血大鼠神经保护作用机制。方法选择SD大鼠60只，脑出血造模成功后随机分为MBP组、磷酸盐缓冲盐水（ PBS ）组、卵白蛋白（ OVA）组，每组30只。每天应用Longa五级评分法进行神经功能缺损评分，不同亚组按相应时间点取材，分别测定出血周围脑组织胶质纤维酸性蛋白（ GFAP）和脑源性神经营养因子（BDNF）的免疫组化评分。取脾组织制作匀浆，反转录聚合酶链式反应（RT-PCR）测定Foxp3与β-actin的相对密度值。结果 MBP组在对应时间点神经功能缺损评分及GFAP和BDNF的表达均明显高于OVA组和PBS组（P＜0．05或0．01）；OVA组与PBS组比较均无显著性差异。 Foxp3 mRNA在大鼠脾脏中的表达MBP组均明显低于OVA组和PBS组（ P均＜0.01），OVA组与 PBS组比较无显著性差异。结论 MBP对实验性脑出血后大鼠的行为学缺损有治疗作用，可减轻症状，上调BDNF、GFAP蛋白表达水平，降低Foxp3 mRNA的表达；对血肿周边神经元产生保护作用，促进中枢神经系统损伤的恢复。
목적：연구수초감성단백（ MBP）대실험성뇌출혈대서신경보호작용궤제。방법선택SD대서60지，뇌출혈조모성공후수궤분위MBP조、린산염완충염수（ PBS ）조、란백단백（ OVA）조，매조30지。매천응용Longa오급평분법진행신경공능결손평분，불동아조안상응시간점취재，분별측정출혈주위뇌조직효질섬유산성단백（ GFAP）화뇌원성신경영양인자（BDNF）적면역조화평분。취비조직제작균장，반전록취합매련식반응（RT-PCR）측정Foxp3여β-actin적상대밀도치。결과 MBP조재대응시간점신경공능결손평분급GFAP화BDNF적표체균명현고우OVA조화PBS조（P＜0．05혹0．01）；OVA조여PBS조비교균무현저성차이。 Foxp3 mRNA재대서비장중적표체MBP조균명현저우OVA조화PBS조（ P균＜0.01），OVA조여 PBS조비교무현저성차이。결론 MBP대실험성뇌출혈후대서적행위학결손유치료작용，가감경증상，상조BDNF、GFAP단백표체수평，강저Foxp3 mRNA적표체；대혈종주변신경원산생보호작용，촉진중추신경계통손상적회복。
Objectiev It is to study the mechanism of nerve protection of myelin basic protein ( MBP) in the rats with ex-perimental intracerebral hemorrhage .Methods 60 SD rats were selected and were randomly divided into MBP group , phos-phate buffered solution ( PBS) group and ovalbumin ( OVA) group after intracerebral hemorrhage models were successfully es-tablished, each group had 30 rats.Neurological deficit scores were evaluated every day by Longa 5 degree scores.All the mod-el rats were drawn the materials on the corresponding time .The expression of GFAP and BDNF in the brain tissue was detected with immuno-histochemistry .Homogenate was made from spleen to detect the relative light density of Foxp 3 and β-action by RT-PCR.Results Neurological deficit scores on the corresponding time and the expressions of GFAP and BDNF in MBP group were higher than those in OVA group and PBS group (P<0.05 or 0.01), but there was no significant difference in the indexes above between OVA group and PBS group .The expression of Foxp3 mRNA in the rats’ spleen tissue was obviously lower than that in OVA group and PBS group , but no significant difference was found between the latter two groups .Conclu-sion MBP is beneficial to improving the behavioral deficit in experimental intracerebral hemorrhage rats , which can relieve symptoms, increase the expression of BDNF and GFAP , decrease the expression of Foxp3mRNA,thus to protect the nerve cells around the hematoma and promote the recovery of central nervous system injury .