CAJ | 학술논문

目的：探讨膝关节骨关节炎（ OA）患者印度刺猬蛋白（ Ihh）与骨关节炎的相关性。方法2010年3月至2012年6月山西省长治市长治医学院附属和平医院骨科215例膝关节OA患者，男97例，女118例；年龄49～78（65.2±7.8）岁；34例健康对照者，男28例，女6例，年龄45～62（58.4±8.7）岁。收集膝关节软骨标本，采用改良的Mankin评分评价疾病的严重程度，实时荧光定量聚合酶链反应法测定 Ihh mRNA 的量，免疫印迹法测定 Ihh 蛋白表达水平。结果根据改良的Mankin评分，将膝关节骨关节炎标本分为重度、中度、轻度3个级别。与正常对照组比较，骨关节炎软骨Ihh mRNA和蛋白表达均明显升高。而且随着软骨破坏度加重，Ihh mRNA和蛋白进一步增加，各组结果差异有统计学意义。 Ihh mRNA：正常组[n ＝34，（1.00±0.01）]、轻度损伤组[n＝67，（1.37±0.02）]、中度损伤组[n＝63，（1.90±0.02）]和重度损伤组[n＝85，（2.13±0.03）]（均P＜0.01）。膝关节OA的严重程度由膝关节软组织的损伤程度（ Mankin评分）判断；膝关节软骨损伤程度和Ihh表达呈正相关（ r＝0.581， P＜0.01）。结论 Ihh的表达与膝关节骨关节炎病变过程，软骨细胞的形态改变相关。
목적：탐토슬관절골관절염（ OA）환자인도자위단백（ Ihh）여골관절염적상관성。방법2010년3월지2012년6월산서성장치시장치의학원부속화평의원골과215례슬관절OA환자，남97례，녀118례；년령49～78（65.2±7.8）세；34례건강대조자，남28례，녀6례，년령45～62（58.4±8.7）세。수집슬관절연골표본，채용개량적Mankin평분평개질병적엄중정도，실시형광정량취합매련반응법측정 Ihh mRNA 적량，면역인적법측정 Ihh 단백표체수평。결과근거개량적Mankin평분，장슬관절골관절염표본분위중도、중도、경도3개급별。여정상대조조비교，골관절염연골Ihh mRNA화단백표체균명현승고。이차수착연골파배도가중，Ihh mRNA화단백진일보증가，각조결과차이유통계학의의。 Ihh mRNA：정상조[n ＝34，（1.00±0.01）]、경도손상조[n＝67，（1.37±0.02）]、중도손상조[n＝63，（1.90±0.02）]화중도손상조[n＝85，（2.13±0.03）]（균P＜0.01）。슬관절OA적엄중정도유슬관절연조직적손상정도（ Mankin평분）판단；슬관절연골손상정도화Ihh표체정정상관（ r＝0.581， P＜0.01）。결론 Ihh적표체여슬관절골관절염병변과정，연골세포적형태개변상관。
Objective To explore the correlation between the Indian hedgehog and severity of knee osteoarthritis cartilage damage.Methods A total of 215 patients with osteoarthritis [ 97 males and 118 females with an average age of 65.2 ±7.8 (49-78) years and 34 healthy persons [28 males and 6 females with an average age of 58.4 ±8.7 (45-62) years were selected randomly and their cartilages in knee joints collected.The damage degree of osteoarthritis was evaluated by revised Markin scale.The Ihh mRNA was assessed by real-time polymerase chain reaction ( PCR) and the Ihh protein expression examined by Western blot.Results According to the revised Mankin scale , the osteoarthritis samples were divided into 3 stages of severe, moderate and mild.Comparing with normal control group , the Ihh mRNA and protein expressions increased obviously in cartilage.With worsening cartilage damage , Ihh mRNA and protein expression increased accordingly.Statistical differences existed among the groups ( normal:1.00 ±0.01;mild:1.37 ± 0.02;moderate:1.90 ±0.02;severe:2.13 ±0.03; P<0.01 ).And the damage degree of osteoarthritis was determined by injury extent of soft tissue in knee joint ( Mankin scale ).And the damage degree of cartilage was positively correlated with Ihh expression (r=0.581, P<0.01).Conclusion Ihh expression is correlated with the damage degree of knee joint osteoarthritis.