CAJ | 학술논문

目的：探讨扩大电化学免疫传感器在检测食品及饲料中黄曲霉毒素B1（A FB1）的应用范围，分析抗体孵育时间及各种前处理方法对检测结果的影响。方法利用A FB1和羧基化单壁碳纳米管构建的双层免疫传感器，采用循环伏安法对传感器进行验证，研究了抗体孵育时间和样品前处理方法对检测结果的影响。结果 AFB1抗体和二抗的最佳孵育时间为90 min ，且不同的前处理方法对检测结果影响较大，粗提溶液经过A FB1亲和柱纯化、浓缩能够有效排除样品基质干扰物对测定结果的影响。结论电化学免疫传感器检测A FB1具有快速、简便、检测限低等特点，其稳定性受到抗体孵育时间以及样品前处理等因素影响。
목적：탐토확대전화학면역전감기재검측식품급사료중황곡매독소B1（A FB1）적응용범위，분석항체부육시간급각충전처리방법대검측결과적영향。방법이용A FB1화최기화단벽탄납미관구건적쌍층면역전감기，채용순배복안법대전감기진행험증，연구료항체부육시간화양품전처리방법대검측결과적영향。결과 AFB1항체화이항적최가부육시간위90 min ，차불동적전처리방법대검측결과영향교대，조제용액경과A FB1친화주순화、농축능구유효배제양품기질간우물대측정결과적영향。결론전화학면역전감기검측A FB1구유쾌속、간편、검측한저등특점，기은정성수도항체부육시간이급양품전처리등인소영향。
Objective To expand the application of electrochemical immunosensor during deleting aflatoxin B1 in foods and feeds through analyzing impacts of the time of antibody incubation and sample preparation .Methods T he double self-assembly immu-nosensor combined with aflatoxin B1 and carboxylated single-walled carbon nanotubes (SWNTs) was characterized by cyclic volta-mmetry and impacts of the time of antibody incubation and sample preparation methods were investigated .Results The signal in-creased gradually following the increasing time of antibody incubation and reached a plateau at 90 min and sample preparation meth-ods showed a comparatively large impact on results .Additionally ,the crude extractions purified through removing interfering com-pounds by immunoaffinity column could effectively eliminate the interference effects of sample matrix .Conclusion Deleting aflatox-in B1 by electrochemical immunosensor is characterized by various features ,such as fast ,simple and low detection limits .The pres-ent study shows that stability of the electrochemical immunosensor is affected by the time of antibody incubation and sample prepa-ration .