CAJ | 학술논문

目的：观察超深低温（-196℃）条件下不同冷冻时间对异体神经移植后生理特性的影响。方法将80只雌性Wis tar大鼠随机分为取材组（20只），对照组（A组：新鲜自体神经移植组；B组：新鲜异体神经移植组，每组10只）和实验组（按取材神经冷冻保存3、6、9、12周后移植分为C、D、E、F组，每组10只）。术后做大体、光镜、电镜观察，神经电生理测试。结果移植9周后，大体、光镜、电镜结果显示：B组生理特性影响最大，A组最小，C、D、E、F组次之；电生理结果显示：A与B、A与E、A与F之间差异有统计学意义（P＜0．05）。结论异体神经移植后的生理特性和冷冻时间存在依从关系。
목적：관찰초심저온（-196℃）조건하불동냉동시간대이체신경이식후생리특성적영향。방법장80지자성Wis tar대서수궤분위취재조（20지），대조조（A조：신선자체신경이식조；B조：신선이체신경이식조，매조10지）화실험조（안취재신경냉동보존3、6、9、12주후이식분위C、D、E、F조，매조10지）。술후주대체、광경、전경관찰，신경전생리측시。결과이식9주후，대체、광경、전경결과현시：B조생리특성영향최대，A조최소，C、D、E、F조차지；전생리결과현시：A여B、A여E、A여F지간차이유통계학의의（P＜0．05）。결론이체신경이식후적생리특성화냉동시간존재의종관계。
Objective To observe the effect of different conserve time in ultra deep cryopreservation (-196 ℃)on physiological characteristics of allograft nerve transplantation .Methods A total of 80 female Wistar rats were divide into three groups by random principle:derived group which 20 rats were sacrificed to get both sides of femoral nerves ;control groups which each group had 10 rats include group A for fresh autologous nerve transplantation ,group B for fresh allograft nerve transplantation ;experiment groups which each group had 10 rats including groups C ,D ,E ,F that transplanted after femoral nerves conserve in the -196 ℃ for 3 ,6 ,9 , 12 weeks respectively ,and the results of exterior appearance ,light microscope ,electron microscope were observed and electrophysi-ological test was conducted after transplantation .Results After 9 weeks transplantation :the physiological characteristics of group B was most affected ,followed by group C ,D ,E ,F ,group A was with minimal impact ;the result of electrophysiological test showed that groups A&B ,A&E ,A&F had significant difference(P<0 .05) .Conclusion The physiological characteristics of allograft nerve transplantation relate to the freezing time .