CAJ | 학술논문

目的：研究不同浓度葡萄糖对神经膜细胞早期生长反应蛋白2（EGR2）表达产生的影响。方法将神经膜细胞株用两种不同浓度的葡萄糖培养后，分组为正常葡萄糖浓度组（5．5 mmol/L ，N组）和高糖组（25 mmol/L ，H组），用免疫细胞化学法和蛋白质印迹法研究EGR2蛋白表达情况。结果 H组EGR2蛋白表达比N组高，差异有统计学意义（ P＜0．05）。结论高糖导致神经膜细胞株EGR2蛋白表达升高，EGR2可能在糖尿病导致的神经病变中发挥作用。
목적：연구불동농도포도당대신경막세포조기생장반응단백2（EGR2）표체산생적영향。방법장신경막세포주용량충불동농도적포도당배양후，분조위정상포도당농도조（5．5 mmol/L ，N조）화고당조（25 mmol/L ，H조），용면역세포화학법화단백질인적법연구EGR2단백표체정황。결과 H조EGR2단백표체비N조고，차이유통계학의의（ P＜0．05）。결론고당도치신경막세포주EGR2단백표체승고，EGR2가능재당뇨병도치적신경병변중발휘작용。
Objective To investigate the effects of different glucose concentration on EGR2 protein expression in Schwann cells . Methods Schwann cells were cultured in different glucose concentrations ,and they were divided into two groups ,normal glucose concentration (5 .5 mmol/L ,N group) and high glucose concentration(25 mmol/L ,H group) .Immunocytochemistry and Western blot were used to study EGR2 expression .Results The expression of EGR2 protein was higher in H group than that in N group the difference was statistically significont (P<0 .05) .Conclusion High glucose can lead to EGR2 protein upregulate in Schwann cells ,and EGR2 maybe one of the reasons of diabetic neuropathy .