中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2010年
2期
136-139
,共4页
张育才%左文琼%龚小慧%滕国良%张宇呜
張育纔%左文瓊%龔小慧%滕國良%張宇嗚
장육재%좌문경%공소혜%등국량%장우오
糖皮质激素%糖皮质激素受体mRNA%血管活性肠肽%脂多糖%休克%炎症%肺损伤%鼠
糖皮質激素%糖皮質激素受體mRNA%血管活性腸肽%脂多糖%休剋%炎癥%肺損傷%鼠
당피질격소%당피질격소수체mRNA%혈관활성장태%지다당%휴극%염증%폐손상%서
Ghcocorticoid%GR mRNA%Vasoactive intestinal peptide%LPS%Shock%Inflammation%Lung injury%Rat
目的 探讨内毒素(LPS)性休克大鼠肺组织糖皮质激素受体(GR)mRNA的表达.方法 56只SD大鼠,随机(随机数字法)分IPS休克组(16只)、LPS+血管活性肠肽(vasosctive intestinal peptide,VIP)组(16只)、LPS+VIP+糖皮质激素(GC)组(16只)和对照组(8只).尾静脉注射LPS制作休克模型,尾静脉注射LPS 10 mg/kg后,分别于15 min内注射VIP 5 nmol/kg或VIP 5 nmol/ks+甲基强的松龙3 mg/kg,对照组注射等容量生理盐水,分别于沣射后6 h和24 h处死,留取肺标本,观察肺组织病理变化,RT-PCR榆测肺组织GR mRNA的表达.结果 (1)肺组织病理改变:LPS组见肺泡腔结构破坏、肺泡间隔增宽、炎性细胞浸润、出血、间质水肿,细胞器破坏,LPS+VIP组和IPS+VIP+GC组病变较轻.(2)GR mRNA的表达:注射LPS后6 h,LPS组和LPs+VIP组GR mRNA表达下降,与对照组相比,差异具有统计学意义(P＜0.05).LPS组下降较LPS+VIP组稍明显,但差异无统计学意义(P＞0.05).24 h GR mRNA表达恢复.LPS+VIP+GC组GR mRNA表达6 h增加,24 h GR mRNA表达更高(P＜0.05).结论 LPS致肺损伤时,肺组织GR mRNA的表达减少.VIP和GC可抵抗炎症反应、减轻肺损伤,其机制可能与增强GR mRNA的表达有关.
目的 探討內毒素(LPS)性休剋大鼠肺組織糖皮質激素受體(GR)mRNA的錶達.方法 56隻SD大鼠,隨機(隨機數字法)分IPS休剋組(16隻)、LPS+血管活性腸肽(vasosctive intestinal peptide,VIP)組(16隻)、LPS+VIP+糖皮質激素(GC)組(16隻)和對照組(8隻).尾靜脈註射LPS製作休剋模型,尾靜脈註射LPS 10 mg/kg後,分彆于15 min內註射VIP 5 nmol/kg或VIP 5 nmol/ks+甲基彊的鬆龍3 mg/kg,對照組註射等容量生理鹽水,分彆于灃射後6 h和24 h處死,留取肺標本,觀察肺組織病理變化,RT-PCR榆測肺組織GR mRNA的錶達.結果 (1)肺組織病理改變:LPS組見肺泡腔結構破壞、肺泡間隔增寬、炎性細胞浸潤、齣血、間質水腫,細胞器破壞,LPS+VIP組和IPS+VIP+GC組病變較輕.(2)GR mRNA的錶達:註射LPS後6 h,LPS組和LPs+VIP組GR mRNA錶達下降,與對照組相比,差異具有統計學意義(P＜0.05).LPS組下降較LPS+VIP組稍明顯,但差異無統計學意義(P＞0.05).24 h GR mRNA錶達恢複.LPS+VIP+GC組GR mRNA錶達6 h增加,24 h GR mRNA錶達更高(P＜0.05).結論 LPS緻肺損傷時,肺組織GR mRNA的錶達減少.VIP和GC可牴抗炎癥反應、減輕肺損傷,其機製可能與增彊GR mRNA的錶達有關.
목적 탐토내독소(LPS)성휴극대서폐조직당피질격소수체(GR)mRNA적표체.방법 56지SD대서,수궤(수궤수자법)분IPS휴극조(16지)、LPS+혈관활성장태(vasosctive intestinal peptide,VIP)조(16지)、LPS+VIP+당피질격소(GC)조(16지)화대조조(8지).미정맥주사LPS제작휴극모형,미정맥주사LPS 10 mg/kg후,분별우15 min내주사VIP 5 nmol/kg혹VIP 5 nmol/ks+갑기강적송룡3 mg/kg,대조조주사등용량생리염수,분별우풍사후6 h화24 h처사,류취폐표본,관찰폐조직병리변화,RT-PCR유측폐조직GR mRNA적표체.결과 (1)폐조직병리개변:LPS조견폐포강결구파배、폐포간격증관、염성세포침윤、출혈、간질수종,세포기파배,LPS+VIP조화IPS+VIP+GC조병변교경.(2)GR mRNA적표체:주사LPS후6 h,LPS조화LPs+VIP조GR mRNA표체하강,여대조조상비,차이구유통계학의의(P＜0.05).LPS조하강교LPS+VIP조초명현,단차이무통계학의의(P＞0.05).24 h GR mRNA표체회복.LPS+VIP+GC조GR mRNA표체6 h증가,24 h GR mRNA표체경고(P＜0.05).결론 LPS치폐손상시,폐조직GR mRNA적표체감소.VIP화GC가저항염증반응、감경폐손상,기궤제가능여증강GR mRNA적표체유관.
Objective To investigate the effects of vasoactive intestinal peptide(VIP)on TLR 2,4 mRNA expressions in the lung tissue in endotoxic shock rat induced by LPS rat.Method Fifty six SD rats were far.rely (random number)divided into 4 groups,including LPS shock group(n=16),LPS+VIP group(n=16),LPS+V1P+GC group(n=16),and control group(n=8).LPS shock model was made by intravenously injecting with LPS(10 mg/kg);rats in the LPS+VIP group were intravenously injected with VIP after LPS;rats in the LPS+VIP+GC group were injected with VIP and methylprednisolone together with LPS;rats in the control group were given with normal saune.The rats were sacrificed at 6 h,24 h after injected.The lung tissues were collected.Pathological changes of the lungs were observed under hght microscope and electron microscope.The GR mRNA expressions were assessed by RT-PCR in the lung tissues.Results(1)Lung histopathology:necrotic abscission cells increased in alveolar space,inflammatory cells infiltration and lung interstitial edema appeared in LPS shock group.However,pathological changes in LPS+VIP group and LPS+VIP+GC group were milder than those in LPS shock group.(2)After 6 hours LPS injection,the GR mRNA expression in LPS group and LPS+VIP group were significantly lower as compared with those of the control group(P＜0.05),the GR mRNA expressions of LPS+VIP+GC group at 6 h and 24 h were significantly higher than those of the other groups(P＜0.05).Condusions Expression of GR mRNA decreases in rats with LPS-induced lung injury.VIP+GC can reduce lung injury,which may have correlation with up-regulation of GR mRNA expression.
