按摩与康复医学
按摩與康複醫學
안마여강복의학
Chinese Manipulation & Rehabilitation Medicine
2014年
4期
213-215
,共3页
杜仲苷%软骨细胞%IL-1β%炎性环境%增殖%II型胶原蛋白
杜仲苷%軟骨細胞%IL-1β%炎性環境%增殖%II型膠原蛋白
두중감%연골세포%IL-1β%염성배경%증식%II형효원단백
aucubin%cartilage cells%IL-1β%inflammatory environment%proliferation%collagen type II
目的:观察杜仲苷对IL-1β刺激大鼠体外软骨细胞的增殖及II型胶原蛋白分泌的影响。方法:利用IL-1β刺激建立体外大鼠软骨细胞炎性模型,实验分为空白组、IL-1β组、10μM杜仲苷组、100μM杜仲苷组、500μM杜仲苷组、1000μM杜仲苷组;空白组加入10%FBS培养液,IL-1β组加入10ng/mL IL-1β+10%FBS培养液,4个不同浓度杜仲苷组分别加入10μM、100μM、500μM、1000μM杜仲苷+10ng/mL IL-1β+10%FBS培养液,分别培养48小时,采用CCK8检测软骨细胞增殖活力及II型胶原蛋白的分泌。结果:IL-1β组的OD值低于空白组(P<0.05);4个不同浓度杜仲苷组的OD值均高于IL-1β组(P<0.05),且存在一定的量效关系;4个不同浓度杜仲苷组的II型胶原蛋白表达均高于IL-1β组(P<0.05),且存在有一定的量效关系。结论:杜仲苷可以提高体外大鼠软骨细胞炎性环境下的增殖活力,促进II型胶原蛋白的分泌,表明杜仲苷对体外大鼠软骨细胞有一定的抗炎保护作用。
目的:觀察杜仲苷對IL-1β刺激大鼠體外軟骨細胞的增殖及II型膠原蛋白分泌的影響。方法:利用IL-1β刺激建立體外大鼠軟骨細胞炎性模型,實驗分為空白組、IL-1β組、10μM杜仲苷組、100μM杜仲苷組、500μM杜仲苷組、1000μM杜仲苷組;空白組加入10%FBS培養液,IL-1β組加入10ng/mL IL-1β+10%FBS培養液,4箇不同濃度杜仲苷組分彆加入10μM、100μM、500μM、1000μM杜仲苷+10ng/mL IL-1β+10%FBS培養液,分彆培養48小時,採用CCK8檢測軟骨細胞增殖活力及II型膠原蛋白的分泌。結果:IL-1β組的OD值低于空白組(P<0.05);4箇不同濃度杜仲苷組的OD值均高于IL-1β組(P<0.05),且存在一定的量效關繫;4箇不同濃度杜仲苷組的II型膠原蛋白錶達均高于IL-1β組(P<0.05),且存在有一定的量效關繫。結論:杜仲苷可以提高體外大鼠軟骨細胞炎性環境下的增殖活力,促進II型膠原蛋白的分泌,錶明杜仲苷對體外大鼠軟骨細胞有一定的抗炎保護作用。
목적:관찰두중감대IL-1β자격대서체외연골세포적증식급II형효원단백분비적영향。방법:이용IL-1β자격건입체외대서연골세포염성모형,실험분위공백조、IL-1β조、10μM두중감조、100μM두중감조、500μM두중감조、1000μM두중감조;공백조가입10%FBS배양액,IL-1β조가입10ng/mL IL-1β+10%FBS배양액,4개불동농도두중감조분별가입10μM、100μM、500μM、1000μM두중감+10ng/mL IL-1β+10%FBS배양액,분별배양48소시,채용CCK8검측연골세포증식활력급II형효원단백적분비。결과:IL-1β조적OD치저우공백조(P<0.05);4개불동농도두중감조적OD치균고우IL-1β조(P<0.05),차존재일정적량효관계;4개불동농도두중감조적II형효원단백표체균고우IL-1β조(P<0.05),차존재유일정적량효관계。결론:두중감가이제고체외대서연골세포염성배경하적증식활력,촉진II형효원단백적분비,표명두중감대체외대서연골세포유일정적항염보호작용。
Objective:To observe the influence of aucubin on the proliferation of vitro cartilage cells from IL-1βstimulated rats and their secretion of collagen type II. Methods:Using IL-1βstimulation to establish inflammatory model of vitro cartilage cells with rats, then divided into control group, IL-1β group, 10μMaucubin group, 100μMaucubin group, 500μMaucubin group and 1000μMaucubin group; 10% FBS medium was put in control group, while 10ng/mL IL-1β+10%FBS medium were in IL-1βgroup, and 10μM、100μM、500μM、1000μMaucubin+10ng/mL IL-1β+10%FBS medi-um were respectively in the four different concentration aucubin groups, all the groups were cultured for 48 hours, and used CCK8 to test prolifera-tion activity of cartilage cells and secretion of collagen type II. Results:The OD value of IL-1βgroup was lower than that of control group (P<0.05);the OD value of four different concetration aucubin groups were all higher than that of IL-1βgroup (P<0.05), and existed a dose-response relation-ship;the expression of collagen type II in the four different concentration aucubin groups were all higher than that of IL-1βgroup (P<0.05), and exist-ed a dose-response relationship. Conclusion:Aucubin can improve proliferation activity of rats' vitro cartilage cells under inflammatory environment, promote the secretion of collagen type II, which indicates that aucubin has certain anti-inflammatory effect on rats' vitro cartilage cells.