按摩与康复医学
按摩與康複醫學
안마여강복의학
Chinese Manipulation & Rehabilitation Medicine
2014年
4期
210-212
,共3页
吴少鹏%孙正平%邓崇礼%李参天%梁灿德
吳少鵬%孫正平%鄧崇禮%李參天%樑燦德
오소붕%손정평%산숭례%리삼천%량찬덕
补肾活血方%骨质疏松症%成骨细胞%p38 MAPK%信号通路%作用机制
補腎活血方%骨質疏鬆癥%成骨細胞%p38 MAPK%信號通路%作用機製
보신활혈방%골질소송증%성골세포%p38 MAPK%신호통로%작용궤제
Bushenhuoxue decoction%osteoporosis%osteoblasts%p38 MAPK%signal pathway%mechanism
目的：观察补肾活血方含药血清对人成骨细胞功能及其对p38 MAPK信号转导通路的活性影响，探讨补肾活血方防治骨质疏松症的作用机制。方法：将人成骨细胞株分为Control组、补肾活血方含药血清组（Z组）、含药血清+p38抑制剂SB203580组（Z+SB组），采用四甲基偶氮唑盐比色法（MTT）检测各组成骨细胞的增殖情况，通过PNPP法分析检测成骨细胞碱性磷酸酶（ALP）的活性，采用免疫印迹（Western blot）法检测和磷酸化p38（p-p38）的表达水平。结果：补肾活血方含药血清能刺激成骨细胞的增殖，使分化增强，与Control组比较有显著性差异（P<0.01）；阻断p38 MAPK信号转导通路后，与Z组比较，对成骨细胞的增殖无明显抑制（P>0.05），而对成骨细胞的分化功能有显著抑制作用（P<0.01）；Western blot结果显示，补肾活血方含药血清能刺激p-p38表达，阻断p38 MAPK信号转导通路后，p-p38含量明显减少（P<0.01）。结论：补肾活血方可能通过p38 MAPK通路调控促进成骨细胞分化的功能，起到防治骨质疏松症的作用。
目的：觀察補腎活血方含藥血清對人成骨細胞功能及其對p38 MAPK信號轉導通路的活性影響，探討補腎活血方防治骨質疏鬆癥的作用機製。方法：將人成骨細胞株分為Control組、補腎活血方含藥血清組（Z組）、含藥血清+p38抑製劑SB203580組（Z+SB組），採用四甲基偶氮唑鹽比色法（MTT）檢測各組成骨細胞的增殖情況，通過PNPP法分析檢測成骨細胞堿性燐痠酶（ALP）的活性，採用免疫印跡（Western blot）法檢測和燐痠化p38（p-p38）的錶達水平。結果：補腎活血方含藥血清能刺激成骨細胞的增殖，使分化增彊，與Control組比較有顯著性差異（P<0.01）；阻斷p38 MAPK信號轉導通路後，與Z組比較，對成骨細胞的增殖無明顯抑製（P>0.05），而對成骨細胞的分化功能有顯著抑製作用（P<0.01）；Western blot結果顯示，補腎活血方含藥血清能刺激p-p38錶達，阻斷p38 MAPK信號轉導通路後，p-p38含量明顯減少（P<0.01）。結論：補腎活血方可能通過p38 MAPK通路調控促進成骨細胞分化的功能，起到防治骨質疏鬆癥的作用。
목적：관찰보신활혈방함약혈청대인성골세포공능급기대p38 MAPK신호전도통로적활성영향，탐토보신활혈방방치골질소송증적작용궤제。방법：장인성골세포주분위Control조、보신활혈방함약혈청조（Z조）、함약혈청+p38억제제SB203580조（Z+SB조），채용사갑기우담서염비색법（MTT）검측각조성골세포적증식정황，통과PNPP법분석검측성골세포감성린산매（ALP）적활성，채용면역인적（Western blot）법검측화린산화p38（p-p38）적표체수평。결과：보신활혈방함약혈청능자격성골세포적증식，사분화증강，여Control조비교유현저성차이（P<0.01）；조단p38 MAPK신호전도통로후，여Z조비교，대성골세포적증식무명현억제（P>0.05），이대성골세포적분화공능유현저억제작용（P<0.01）；Western blot결과현시，보신활혈방함약혈청능자격p-p38표체，조단p38 MAPK신호전도통로후，p-p38함량명현감소（P<0.01）。결론：보신활혈방가능통과p38 MAPK통로조공촉진성골세포분화적공능，기도방치골질소송증적작용。
Objective:To observe the effect of Bushenhuoxue decoction medicated serum on human osteoblasts function and activity of p38 MAPK signal transduction pathway, and investigate the mechanism of the preventing and treating osteoporosis by Bushenhuoxue decoction. Methods:Divid-ed human osteoblasts strains into Control group, Bushenhuoxue decoction medicated serum group (group Z), medicated serum+p38 inhibitor SB203580 group (group Z+SB), used MTT to test the proliferation of osteoblasts in each group, analyzed the activity of osteoblasts' ALP by PNPP, and tested the expression level of p-p38 by Western blot. Results:Bushenhuoxue decoction medicated serum could stimulate the proliferation of os-teoblasts and make its differentiation being enhanced, there were significant differences that compared with Control group (P<0.01);after blocking p38 MAPK signal transduction pathway, compared with group Z, the proliferation of osteoblasts had no significantly restrained (P>0.05), but there was significant inhibition effect on the differentiation of osteoblasts (P<0.01);the Western blot result showed that Bushenhuoxue decoction medicat-ed serum could stimulate the expression of p-p38, and the content of p-p38 would be significantly decreased by blocking p38 MAPK signal transduc-tion pathway (P<0.01). Conclusion:Bushenhuoxue decoction may promote the differentiation of osteoblasts with regulation of p38 NAPK pathway to prevent and cure osteoporosis.
