北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
4期
2-3
,共2页
杜建红%徐小平%王艳丽%郄革红%赵晓娟
杜建紅%徐小平%王豔麗%郄革紅%趙曉娟
두건홍%서소평%왕염려%극혁홍%조효연
稀戊二醛溶液%戊二醛%高效液相色谱法%测定
稀戊二醛溶液%戊二醛%高效液相色譜法%測定
희무이철용액%무이철%고효액상색보법%측정
Glutaraldehyde Dilute%Glutaraldehyde Solution%HPLC%Detemination
目的：建立HPLC法测定稀戊二醛溶液中戊二醛含量的方法。方法：采用AichromBond-AQ C18色谱柱（4.6mm×150mm，5μm），流动相：甲醇∶水（50∶50），流速0.5ml/min，检测波长235nm，进样量20μl。结果：戊二醛在51.6～1010.4μg/ml范围内时，与峰面积呈良好的线性关系（r=0.9992），平均回收率为101.1%。结论：本法操作简便，灵敏度高，重复性好，可用于稀戊二醛溶液中戊二醛的含量控制。
目的：建立HPLC法測定稀戊二醛溶液中戊二醛含量的方法。方法：採用AichromBond-AQ C18色譜柱（4.6mm×150mm，5μm），流動相：甲醇∶水（50∶50），流速0.5ml/min，檢測波長235nm，進樣量20μl。結果：戊二醛在51.6～1010.4μg/ml範圍內時，與峰麵積呈良好的線性關繫（r=0.9992），平均迴收率為101.1%。結論：本法操作簡便，靈敏度高，重複性好，可用于稀戊二醛溶液中戊二醛的含量控製。
목적：건립HPLC법측정희무이철용액중무이철함량적방법。방법：채용AichromBond-AQ C18색보주（4.6mm×150mm，5μm），류동상：갑순∶수（50∶50），류속0.5ml/min，검측파장235nm，진양량20μl。결과：무이철재51.6～1010.4μg/ml범위내시，여봉면적정량호적선성관계（r=0.9992），평균회수솔위101.1%。결론：본법조작간편，령민도고，중복성호，가용우희무이철용액중무이철적함량공제。
Objective:To establish an HPLC method for the determination of Glutaraldehyde in Dilute Glutaraldehyde Solution.Method:An AichromBond-AQ C18 column(4.6mm×150mm,5μm) was used,the mobile phase was Methanol-Water(50∶50).The flow rate was 0.5 ml/min,the detection wavelength was 235nm and the injection volume was 20μl. Results: Within the range from 51.6 to 1010.4μg/ml,Glutaraldehyde presented a good relationship between the injected amounts and peak areas (r=0.9992).The average recovery was 101.1%.Conclusion: The method was easy to operate, with sensitive,and reproducible. It can be used for the quality control of Glutaraldehyde in Dilute Glutaraldehyde Solution.
